Desmoplakin Kit ELISA

N° du produit ABIN5653640

Détail du produit Desmoplakin Kit ELISA

Antigène: Desmoplakin (DSP)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Desmoplakin (DSP). No significant cross-reactivity or interference between Desmoplakin (DSP) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmoplakin (DSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmoplakin (DSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmoplakin (DSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Desmoplakin (DSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Desmoplakin (DSP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Desmoplakin (DSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Desmoplakin (DSP)

Autre désignation: Desmoplakin (DSP Produits)

Synonymes: DP Kit ELISA, DPI Kit ELISA, DPII Kit ELISA, DSP Kit ELISA, dpi Kit ELISA, dpii Kit ELISA, 2300002E22Rik Kit ELISA, 5730453H04Rik Kit ELISA, AA407887 Kit ELISA, AA407888 Kit ELISA, AW109828 Kit ELISA, im:6911953 Kit ELISA, sb:cb570 Kit ELISA, wu:fc17a08 Kit ELISA, wu:fk73d01 Kit ELISA, desmoplakin Kit ELISA, Desmoplakin Kit ELISA, ORF113 Kit ELISA, desmoplakin a Kit ELISA, ORF112 DESMOPLAKIN Kit ELISA, orf102 Kit ELISA, orf91; similar to Cydia pomonella granulovirus orf112 Kit ELISA, ORF62 Kit ELISA, ORF-66 Kit ELISA, ORF57 Kit ELISA, agip105 Kit ELISA, similar to XcORF133; similar to HaGV desmoplakin Kit ELISA, desmoplakin L homeolog Kit ELISA, ORF96 Kit ELISA, DSP Kit ELISA, PhopGV104 Kit ELISA, ORF-59 desmoplakin Kit ELISA, desmop Kit ELISA, Dsp Kit ELISA, dsp Kit ELISA, desmoplakin Kit ELISA, dspa Kit ELISA, orf112 desmoplakin Kit ELISA, ClgVgp102 Kit ELISA, COGV_gp091 Kit ELISA, APNV_p087 Kit ELISA, CBNV_gp066 Kit ELISA, EONV_gp057 Kit ELISA, OLNV_gp066 Kit ELISA, AgipMNPV_gp105 Kit ELISA, HaMNV_gp106 Kit ELISA, EupsNPV_gp065 Kit ELISA, PsunGV_gp139 Kit ELISA, dsp.L Kit ELISA, ClanGV_gp096 Kit ELISA

Sujet:

Gene Name: Desmoplakin

Gene Aliases: DPI, DPII, KPPS2, PPKS2, DP, 250/210 kDa paraneoplastic pemphigus antigen

ID gène: 1832

UniProt: P15924

Pathways: Cell-Cell Junction Organization