DDOST Kit ELISA

N° du produit ABIN5653716

Détail du produit DDOST Kit ELISA

Antigène: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST). No significant cross-reactivity or interference between Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Autre désignation: Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST Produits)

Synonymes: AGER1 Kit ELISA, CDG1R Kit ELISA, OKSWcl45 Kit ELISA, OST Kit ELISA, OST48 Kit ELISA, WBP1 Kit ELISA, DDOST Kit ELISA, wu:fa11d01 Kit ELISA, zgc:66068 Kit ELISA, zgc:77478 Kit ELISA, Ddost Kit ELISA, CG9022 Kit ELISA, DmOST50 Kit ELISA, Dmel\\CG9022 Kit ELISA, DrOST Kit ELISA, OST48/WBP1 Kit ELISA, Ots48 Kit ELISA, anon-EST:Liang-1.11 Kit ELISA, clone 1.11 Kit ELISA, ost48 Kit ELISA, dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit Kit ELISA, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) S homeolog Kit ELISA, dolichyl-diphosphooligosaccharide-protein glycosyltransferase Kit ELISA, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) Kit ELISA, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Kit ELISA, dolichyl-di-phosphooligosaccharide-protein glycotransferase Kit ELISA, Oligosaccharyltransferase 48kD subunit Kit ELISA, DDOST Kit ELISA, ddost.S Kit ELISA, GTR26 Kit ELISA, ddost Kit ELISA, Ddost Kit ELISA, LOC5580056 Kit ELISA, PTRG_01946 Kit ELISA, Ost48 Kit ELISA

Sujet:

Gene Name: Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase

Gene Aliases: AGE-R1, OK/SW-cl.45, OST, OST48, WBP1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, Oligosaccharyl transferase 48 kDa

ID gène: 1650

UniProt: P39656

Pathways: Cell RedoxHomeostasis