DDC Kit ELISA

N° du produit ABIN5653718

Détail du produit DDC Kit ELISA

Antigène: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dopa Decarboxylase (DDC). No significant cross-reactivity or interference between Dopa Decarboxylase (DDC) and analogues was observed.

Sensibilité: 0.61 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopa Decarboxylase (DDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopa Decarboxylase (DDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopa Decarboxylase (DDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopa Decarboxylase (DDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopa Decarboxylase (DDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopa Decarboxylase (DDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Autre désignation: Dopa Decarboxylase (DDC Produits)

Synonymes: AADC Kit ELISA, wu:fa56d05 Kit ELISA, wu:fd59h03 Kit ELISA, wu:fk20h01 Kit ELISA, zgc:65801 Kit ELISA, zgc:76929 Kit ELISA, Ddc Kit ELISA, DdcDv Kit ELISA, Dvir\\GJ17995 Kit ELISA, GJ17995 Kit ELISA, dvir_GLEANR_2561 Kit ELISA, Aadc Kit ELISA, 5-HT Kit ELISA, CG10697 Kit ELISA, DDC Kit ELISA, DdcDm Kit ELISA, Dmel\\CG10697 Kit ELISA, ddc Kit ELISA, fDDC Kit ELISA, l(2)37Bl Kit ELISA, l(2)37Ch Kit ELISA, l(2)k02104 Kit ELISA, dopa decarboxylase Kit ELISA, Dopa-decarboxylase Kit ELISA, dopa decarboxylase (aromatic L-amino acid decarboxylase) Kit ELISA, Dopa decarboxylase Kit ELISA, dopa decarboxylase L homeolog Kit ELISA, DDC Kit ELISA, ddc Kit ELISA, Ddc Kit ELISA, Dvir\Ddc Kit ELISA, ddc.L Kit ELISA

Sujet:

Gene Name: Dopa Decarboxylase

Gene Aliases: AADC, Aromatic L-Amino Acid Decarboxylase, DOPA decarboxylase

ID gène: 1644

UniProt: P20711

Pathways: Dopaminergic Neurogenesis