DBH Kit ELISA

N° du produit ABIN5653721

Détail du produit DBH Kit ELISA

Antigène: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dopamine Beta Hydroxylase (DbH). No significant cross-reactivity or interference between Dopamine Beta Hydroxylase (DbH) and analogues was observed.

Sensibilité: 1.31 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Beta Hydroxylase (DbH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Beta Hydroxylase (DbH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Beta Hydroxylase (DbH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Beta Hydroxylase (DbH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Beta Hydroxylase (DbH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Beta Hydroxylase (DbH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Autre désignation: Dopamine Beta Hydroxylase (DBH Produits)

Synonymes: CG1543 Kit ELISA, DBH Kit ELISA, Dbh Kit ELISA, Dmel\\CG1543 Kit ELISA, T beta h Kit ELISA, TBH Kit ELISA, TBetaH Kit ELISA, TBetah Kit ELISA, TBh Kit ELISA, TbetaH Kit ELISA, TbetaM Kit ELISA, Tbetah Kit ELISA, tbh Kit ELISA, DBM Kit ELISA, DOPBHY Kit ELISA, Tbh Kit ELISA, dopamine beta-hydroxylase (dopamine beta-monooxygenase) Kit ELISA, Tyramine beta hydroxylase Kit ELISA, MOXD1 homolog 2 Kit ELISA, dopamine beta hydroxylase Kit ELISA, dopamine beta-hydroxylase Kit ELISA, dbh Kit ELISA, Tbh Kit ELISA, LOC5574699 Kit ELISA, CpipJ_CPIJ004736 Kit ELISA, CpipJ_CPIJ004737 Kit ELISA, CpipJ_CPIJ014202 Kit ELISA, CpipJ_CPIJ016745 Kit ELISA, CpipJ_CPIJ019034 Kit ELISA, DBH Kit ELISA, Dbh Kit ELISA, LOC101110668 Kit ELISA

Sujet:

Gene Name: Dopamine Beta Hydroxylase

Gene Aliases: DBM, Dopamine Beta-Monooxygenase

ID gène: 1621

UniProt: P09172

Pathways: Carbohydrate Homeostasis