Dystrophin Kit ELISA

N° du produit ABIN5653761

Détail du produit Dystrophin Kit ELISA

Antigène: Dystrophin (DMD)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dystrophin (DMD). No significant cross-reactivity or interference between Dystrophin (DMD) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrophin (DMD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrophin (DMD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrophin (DMD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dystrophin (DMD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dystrophin (DMD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dystrophin (DMD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Dystrophin (DMD)

Autre désignation: Dystrophin (DMD Produits)

Synonymes: DMD Kit ELISA, Dmd Kit ELISA, MGC83347 Kit ELISA, DKFZp459C1629 Kit ELISA, dmd Kit ELISA, MGC79631 Kit ELISA, DKFZp468A1620 Kit ELISA, Dp71 Kit ELISA, cb664 Kit ELISA, zfDYS Kit ELISA, im:6911785 Kit ELISA, zgc:110165 Kit ELISA, BMD Kit ELISA, CMD3B Kit ELISA, DXS142 Kit ELISA, DXS164 Kit ELISA, DXS206 Kit ELISA, DXS230 Kit ELISA, DXS239 Kit ELISA, DXS268 Kit ELISA, DXS269 Kit ELISA, DXS270 Kit ELISA, DXS272 Kit ELISA, DXSmh7 Kit ELISA, DXSmh9 Kit ELISA, Dp427 Kit ELISA, dys Kit ELISA, mdx Kit ELISA, pke Kit ELISA, DNADMD1 Kit ELISA, RATDMD Kit ELISA, CG17750 Kit ELISA, CG31175 Kit ELISA, CG34157 Kit ELISA, CG7240 Kit ELISA, CG7243 Kit ELISA, CG7344 Kit ELISA, DLP Kit ELISA, DLP1 Kit ELISA, DLP186 Kit ELISA, DLP2 Kit ELISA, DLP3 Kit ELISA, DmDLP Kit ELISA, DmDYS Kit ELISA, Dmel\\CG34157 Kit ELISA, Dp117 Kit ELISA, Dp186 Kit ELISA, Dp205 Kit ELISA, GI3046716 Kit ELISA, IDLP Kit ELISA, det Kit ELISA, dmDLP Kit ELISA, dmDp186 Kit ELISA, dmDys Kit ELISA, dystrophin Kit ELISA, dystrophin, gene 1 L homeolog Kit ELISA, dystrophin, gene 1 Kit ELISA, dystrophin, muscular dystrophy Kit ELISA, Dystrophin Kit ELISA, DMD Kit ELISA, LOC708073 Kit ELISA, dmd.1.L Kit ELISA, LOC465557 Kit ELISA, dmd.1 Kit ELISA, dmd Kit ELISA, Dmd Kit ELISA, Dys Kit ELISA

Sujet:

Gene Name: Dystrophin

Gene Aliases: CMD3B, Muscular Dystrophy,Duchenne And Becker Types

ID gène: 1756

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development