Elastase 3B Kit ELISA

N° du produit ABIN5653800

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Elastase 3B.

Aperçu rapide pour Elastase 3B Kit ELISA (ABIN5653800)

Antigène: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Elastase 3B Kit ELISA

Seuil minimal de détection: 0.312 ng/mL

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Elastase 3B (ELA3B). No significant cross-reactivity or interference between Elastase 3B (ELA3B) and analogues was observed.

Sensibilité: 0.135 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3B (ELA3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3B (ELA3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3B (ELA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 3B (ELA3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Autre désignation: Elastase 3B

Sujet:

Gene Name: Elastase 3B

Gene Aliases: CELA3B, Chymotrypsin Like Elastase Family Member 3B, Protease E, Fecal Elastase, Elastase 1, Cholesterol-Binding Pancreatic Protease, Pancreatic Endopeptidase E

ID gène: 23436

UniProt: P08861