EPI Kit ELISA

N° du produit ABIN5653940

Kit ELISA EPI Différentes espèces, Colorimetric test pour la quantification de Différentes espèces EPI.

Aperçu rapide pour EPI Kit ELISA (ABIN5653940)

Antigène: EPI (Epinephrine/Adrenaline (EPI))

Reactivité: Différentes espèces

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 24.69 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum

Détail du produit EPI Kit ELISA

Seuil minimal de détection: 24.69 pg/mL

Fonction: Epinephrine (EPI) ELISA Kit for detecting EPI in serum, plasma, cell lysates, cell culture supernates

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Epinephrine (EPI). No significant cross-reactivity or interference between Epinephrine (EPI) and analogues was observed.

Sensibilité: 9.74 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Epinephrine (EPI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Epinephrine (EPI) and unlabeled Epinephrine (EPI) (Standards or samples) with the pre-coated antibody specific to Epinephrine (EPI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Epinephrine (EPI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Epinephrine (EPI) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epinephrine (EPI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epinephrine (EPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: EPI (Epinephrine/Adrenaline (EPI))

Autre désignation: Epinephrine

Classe de substances: Chemical

Sujet: Gene Aliases: Adrenaline