Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

EPYC Kit ELISA

EPYC Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN5653942
  • Antigène Voir toutes EPYC Kits ELISA
    EPYC (Epiphycan (EPYC))
    Reactivité
    • 4
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Epiphycan (EPYC). No significant cross-reactivity or interference between Epiphycan (EPYC) and analogues was observed.
    Sensibilité
    0.056 ng/mL
    Featured
    Discover our best selling EPYC Kit ELISA
    Top Product
    Discover our top product EPYC Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epiphycan (EPYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epiphycan (EPYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epiphycan (EPYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epiphycan (EPYC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epiphycan (EPYC) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epiphycan (EPYC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes EPYC Kits ELISA
    EPYC (Epiphycan (EPYC))
    Autre désignation
    Epiphycan (EPYC Produits)
    Synonymes
    MGC85488 Kit ELISA, dspg3 Kit ELISA, MGC69472 Kit ELISA, DSPG3 Kit ELISA, im:6901189 Kit ELISA, wu:fb48c08 Kit ELISA, zgc:110596 Kit ELISA, si:dkeyp-38g8.4 Kit ELISA, Dspg3 Kit ELISA, PG-Lb Kit ELISA, SLRR3B Kit ELISA, PGLB Kit ELISA, Pg-Lb Kit ELISA, epiphycan S homeolog Kit ELISA, epiphycan Kit ELISA, epyc.S Kit ELISA, epyc Kit ELISA, EPYC Kit ELISA, Epyc Kit ELISA
    Sujet

    Gene Name: Epiphycan

    Gene Aliases: DSPG3, Pg-Lb, SLRR3B, Dermatan Sulphate Proteoglycan 3, Dermatan Sulfate Proteoglycan 3, Proteoglycan-Lb, Small chondroitin/dermatan sulfate proteoglycan

    ID gène
    1833
    UniProt
    Q99645
Vous êtes ici:
Support technique