EpCAM Kit ELISA

N° du produit ABIN5653952

Détail du produit EpCAM Kit ELISA

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Epithelial Cell Adhesion Molecule (EPCAM). No significant cross-reactivity or interference between Epithelial Cell Adhesion Molecule (EPCAM) and analogues was observed.

Sensibilité: 12.6 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epithelial Cell Adhesion Molecule (EPCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epithelial Cell Adhesion Molecule (EPCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epithelial Cell Adhesion Molecule (EPCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epithelial Cell Adhesion Molecule (EPCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epithelial Cell Adhesion Molecule (EPCAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epithelial Cell Adhesion Molecule (EPCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: Epithelial Cell Adhesion Molecule (EPCAM Produits)

Synonymes: DIAR5 Kit ELISA, EGP-2 Kit ELISA, EGP314 Kit ELISA, EGP40 Kit ELISA, ESA Kit ELISA, HNPCC8 Kit ELISA, KS1/4 Kit ELISA, KSA Kit ELISA, M4S1 Kit ELISA, MIC18 Kit ELISA, MK-1 Kit ELISA, TACSTD1 Kit ELISA, TROP1 Kit ELISA, ECS-1 Kit ELISA, ECS1 Kit ELISA, ESA1 Kit ELISA, M17S1 Kit ELISA, EGP Kit ELISA, Ep-CAM Kit ELISA, sb:cb6 Kit ELISA, tacstd Kit ELISA, wu:fj17g02 Kit ELISA, zgc:110304 Kit ELISA, zgc:77119 Kit ELISA, tacstd1 Kit ELISA, MGC80540 Kit ELISA, EPCAM Kit ELISA, egp Kit ELISA, ksa Kit ELISA, m4s1 Kit ELISA, mk-1 Kit ELISA, cd326 Kit ELISA, egp40 Kit ELISA, mic18 Kit ELISA, trop1 Kit ELISA, ep-cam Kit ELISA, hegp-2 Kit ELISA, co17-1a Kit ELISA, ga733-2 Kit ELISA, CD326 Kit ELISA, Egp314 Kit ELISA, EpCAM1 Kit ELISA, GA733-2 Kit ELISA, Ly74 Kit ELISA, Tacsd1 Kit ELISA, Tacstd1 Kit ELISA, gp40 Kit ELISA, epithelial cell adhesion molecule Kit ELISA, flotillin 2 Kit ELISA, epithelial cell adhesion molecule S homeolog Kit ELISA, EPCAM Kit ELISA, FLOT2 Kit ELISA, epcam Kit ELISA, epcam.S Kit ELISA, Epcam Kit ELISA

Sujet:

Gene Name: Epithelial Cell Adhesion Molecule

Gene Aliases: CD326, TACSTD1, EGP, KSA, M4S1, MIC18, TROP1, HEGP2, ESA, Epithelial glycoprotein, Adenocarcinoma-associated antigen, Tumor Associated Calcium Signal Transducer 1