Estrogen Receptor alpha Kit ELISA

N° du produit ABIN5653989

Détail du produit Estrogen Receptor alpha Kit ELISA

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Alpha (ERa). No significant cross-reactivity or interference between Estrogen Receptor Alpha (ERa) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Alpha (ERa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Alpha (ERa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Alpha (ERa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Alpha (ERa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Alpha (ERa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Alpha (ERa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Autre désignation: Estrogen Receptor Alpha (ESR1 Produits)

Synonymes: LOC398734 Kit ELISA, AA420328 Kit ELISA, AU041214 Kit ELISA, ER-alpha Kit ELISA, ER[a] Kit ELISA, ERa Kit ELISA, ERalpha Kit ELISA, ESR Kit ELISA, Estr Kit ELISA, Estra Kit ELISA, Nr3a1 Kit ELISA, NR3A1 Kit ELISA, eralpha Kit ELISA, zfER[a] Kit ELISA, ER Kit ELISA, ESRA Kit ELISA, ESTRR Kit ELISA, Era Kit ELISA, akap12 Kit ELISA, cb27 Kit ELISA, esr1 Kit ELISA, fb72g12 Kit ELISA, id:ibd1202 Kit ELISA, sb:cb27 Kit ELISA, si:ch73-192g21.1 Kit ELISA, wu:fb72g12 Kit ELISA, wu:fc21c03 Kit ELISA, Esr Kit ELISA, RNESTROR Kit ELISA, ERALPHA Kit ELISA, er Kit ELISA, esr Kit ELISA, nr3a1 Kit ELISA, XER Kit ELISA, era Kit ELISA, esra Kit ELISA, ERbeta Kit ELISA, xesr-1 Kit ELISA, xlERalpha1 Kit ELISA, xlERalpha2 Kit ELISA, ESR1 Kit ELISA, estrogen receptor 1 L homeolog Kit ELISA, estrogen receptor 1 (alpha) Kit ELISA, estrogen receptor 1 Kit ELISA, A kinase (PRKA) anchor protein 12b Kit ELISA, esr1.L Kit ELISA, Esr1 Kit ELISA, ESR1 Kit ELISA, esr1 Kit ELISA, akap12b Kit ELISA

Sujet:

Gene Name: Estrogen Receptor Alpha

Gene Aliases: ER-A1, ER, ESR, ESR1, ESRA, NR3A1, NR3-A1, Estrogen Receptor 1, Nuclear Receptor Subfamily 3,Group A,Member 1

ID gène: 24890

UniProt: P06211

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly