ESR2 Kit CLIA

N° du produit ABIN5653990

Détail du produit ESR2 Kit CLIA

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 312.5 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312.5 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb). No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.

Sensibilité: 10.17 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Estrogen Receptor Beta (ERb) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Autre désignation: Estrogen Receptor Beta (ESR2 Produits)

Synonymes: ER-BETA Kit CLIA, ESR-BETA Kit CLIA, ESRB Kit CLIA, ESTRB Kit CLIA, Erb Kit CLIA, NR3A2 Kit CLIA, ER[b]1 Kit CLIA, zfER-beta1 Kit CLIA, zfER[b]1 Kit CLIA, erb2 Kit CLIA, CERB Kit CLIA, ERB Kit CLIA, erb Kit CLIA, esrb Kit CLIA, estrb Kit CLIA, nr3a2 Kit CLIA, ERbeta Kit CLIA, er-beta Kit CLIA, esr-beta Kit CLIA, ESR2 Kit CLIA, ERBETA Kit CLIA, er Kit CLIA, ER-beta Kit CLIA, ER[b] Kit CLIA, Estrb Kit CLIA, ER[b]2 Kit CLIA, ER[b]a Kit CLIA, zfER[b]2 Kit CLIA, zfER-beta2 Kit CLIA, Erb2 Kit CLIA, esr2 Kit CLIA, erbeta Kit CLIA, Esr2 Kit CLIA, ERBeta2 Kit CLIA, estrogen receptor 2 Kit CLIA, estrogen receptor 2b Kit CLIA, estrogen receptor beta 2 Kit CLIA, estrogen receptor 2 (beta) Kit CLIA, estrogen receptor 2a Kit CLIA, estrogen receptor beta Kit CLIA, ESR2 Kit CLIA, esr2b Kit CLIA, erb2 Kit CLIA, esr2 Kit CLIA, Esr2 Kit CLIA, esr2a Kit CLIA, erbeta Kit CLIA

Sujet:

Gene Name: Estrogen Receptor Beta

Gene Aliases: ER-B, ESRB, ESTRB, NR3A2, NR3-A2, ESR2, Estrogen Receptor 2, Nuclear Receptor Subfamily 3,Group A,Member 2

ID gène: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling