FBXL3 Kit ELISA

N° du produit ABIN5654031

Détail du produit FBXL3 Kit ELISA

Antigène: FBXL3 (F-Box and Leucine-Rich Repeat Protein 3 (FBXL3))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of F-Box And Leucine Rich Repeat Protein 3 (FBXL3). No significant cross-reactivity or interference between F-Box And Leucine Rich Repeat Protein 3 (FBXL3) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to F-Box And Leucine Rich Repeat Protein 3 (FBXL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to F-Box And Leucine Rich Repeat Protein 3 (FBXL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F-Box And Leucine Rich Repeat Protein 3 (FBXL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-Box And Leucine Rich Repeat Protein 3 (FBXL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level F-Box And Leucine Rich Repeat Protein 3 (FBXL3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level F-Box And Leucine Rich Repeat Protein 3 (FBXL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FBXL3 (F-Box and Leucine-Rich Repeat Protein 3 (FBXL3))

Autre désignation: F-Box And Leucine Rich Repeat Protein 3 (FBXL3 Produits)

Synonymes: FBL3 Kit ELISA, FBL3A Kit ELISA, FBXL3A Kit ELISA, AU041772 Kit ELISA, AW212966 Kit ELISA, FBK Kit ELISA, Fbl3a Kit ELISA, Fbxl3a Kit ELISA, Ovtm Kit ELISA, Play68 Kit ELISA, fbxl3 Kit ELISA, FBXL3 Kit ELISA, F7A7.240 Kit ELISA, F7A7_240 Kit ELISA, im:7153024 Kit ELISA, zgc:103721 Kit ELISA, F-box and leucine rich repeat protein 3 Kit ELISA, F-box and leucine-rich repeat protein 3 Kit ELISA, F-box and leucine-rich repeat protein 3 L homeolog Kit ELISA, RNI-like superfamily protein Kit ELISA, F-box and leucine-rich repeat protein 3a Kit ELISA, FBXL3 Kit ELISA, Fbxl3 Kit ELISA, fbxl3.L Kit ELISA, fbxl3 Kit ELISA, AT5G01720 Kit ELISA, fbxl3a Kit ELISA

Sujet:

Gene Name: F-Box And Leucine Rich Repeat Protein 3

Gene Aliases: FBL3, FBL3A, FBXL3A, F-box/LRR-repeat protein 3A

ID gène: 50789

UniProt: Q8C4V4

Pathways: Photoperiodism