Fatty Acid Synthase Kit CLIA

N° du produit ABIN5654091

Détail du produit Fatty Acid Synthase Kit CLIA

Antigène: Fatty Acid Synthase (FASN)

Reactivité: Poulet

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Synthase (FASN). No significant cross-reactivity or interference between Fatty Acid Synthase (FASN) and analogues was observed.

Sensibilité: 2.37 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Synthase (FASN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fatty Acid Synthase (FASN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fatty Acid Synthase (FASN) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Fatty Acid Synthase (FASN)

Autre désignation: Fatty Acid Synthase (FASN Produits)

Synonymes: FAS Kit CLIA, OA-519 Kit CLIA, SDR27X1 Kit CLIA, FASN Kit CLIA, fj34h12 Kit CLIA, wu:fj34h12 Kit CLIA, Cgl0836 Kit CLIA, A630082H08Rik Kit CLIA, BcDNA:GH07626 Kit CLIA, BcDNA:gh07626 Kit CLIA, DM_7295848 Kit CLIA, Dmel\\CG3523 Kit CLIA, Fatty acid synthase Kit CLIA, anon-EST:Posey235 Kit CLIA, anon-EST:Posey43 Kit CLIA, anon-WO0138581.1 Kit CLIA, dFAS Kit CLIA, fas Kit CLIA, fatty acid synthase Kit CLIA, fatty acid synthase Fas Kit CLIA, type I polyketide synthase Kit CLIA, Fatty acid synthase Fas Kit CLIA, Fatty Acid SyNthase Kit CLIA, fatty-acid synthase Kit CLIA, fatty-acid synthase II Kit CLIA, fatty-acid synthase fas Kit CLIA, Fatty acid synthase 1 Kit CLIA, FASN Kit CLIA, fas Kit CLIA, Fasn Kit CLIA, BAD_RS01360 Kit CLIA, fasn Kit CLIA, fasn-1 Kit CLIA, NCgl0802 Kit CLIA, fasB Kit CLIA, MT2600 Kit CLIA, TBMG_01448 Kit CLIA, FASN1 Kit CLIA

Sujet:

Gene Name: Fatty Acid Synthase

Gene Aliases: FAS, OA519, SDR27X1, Short Chain Dehydrogenase/Reductase Family 27X,Member 1, S-acetyltransferase, 3-oxoacyl synthase, Oleoyl hydrolase, Enoyl-acyl-carrier-protein reductase

UniProt: P12276

Pathways: AMPK Signaling