Fatty Acid Synthase Kit ELISA

N° du produit ABIN5654094

Détail du produit Fatty Acid Synthase Kit ELISA

Antigène: Fatty Acid Synthase (FASN)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Synthase (FASN). No significant cross-reactivity or interference between Fatty Acid Synthase (FASN) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Synthase (FASN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Synthase (FASN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Synthase (FASN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Synthase (FASN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Fatty Acid Synthase (FASN)

Autre désignation: Fatty Acid Synthase (FASN Produits)

Synonymes: FAS Kit ELISA, OA-519 Kit ELISA, SDR27X1 Kit ELISA, FASN Kit ELISA, fj34h12 Kit ELISA, wu:fj34h12 Kit ELISA, Cgl0836 Kit ELISA, A630082H08Rik Kit ELISA, BcDNA:GH07626 Kit ELISA, BcDNA:gh07626 Kit ELISA, DM_7295848 Kit ELISA, Dmel\\CG3523 Kit ELISA, Fatty acid synthase Kit ELISA, anon-EST:Posey235 Kit ELISA, anon-EST:Posey43 Kit ELISA, anon-WO0138581.1 Kit ELISA, dFAS Kit ELISA, fas Kit ELISA, fatty acid synthase Kit ELISA, fatty acid synthase Fas Kit ELISA, type I polyketide synthase Kit ELISA, Fatty acid synthase Fas Kit ELISA, Fatty Acid SyNthase Kit ELISA, fatty-acid synthase Kit ELISA, fatty-acid synthase II Kit ELISA, fatty-acid synthase fas Kit ELISA, Fatty acid synthase 1 Kit ELISA, FASN Kit ELISA, fas Kit ELISA, Fasn Kit ELISA, BAD_RS01360 Kit ELISA, fasn Kit ELISA, fasn-1 Kit ELISA, NCgl0802 Kit ELISA, fasB Kit ELISA, MT2600 Kit ELISA, TBMG_01448 Kit ELISA, FASN1 Kit ELISA

Sujet:

Gene Name: Fatty Acid Synthase

Gene Aliases: FAS, OA519, SDR27X1, Short Chain Dehydrogenase/Reductase Family 27X,Member 1, S-acetyltransferase, 3-oxoacyl synthase, Oleoyl hydrolase, Enoyl-acyl-carrier-protein reductase

ID gène: 14104

UniProt: P19096

Pathways: AMPK Signaling