Ferritin Kit ELISA

N° du produit ABIN5654113

Détail du produit Ferritin Kit ELISA

Antigène: Ferritin (FE)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ferritin (FE). No significant cross-reactivity or interference between Ferritin (FE) and analogues was observed.

Sensibilité: 24.7 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin (FE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferritin (FE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin (FE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferritin (FE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin (FE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin (FE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Ferritin (FE)

Autre désignation: Ferritin (FE Produits)

Synonymes: 1HCH Kit ELISA, BEST:LD36673 Kit ELISA, CG2216 Kit ELISA, Dmel\\CG2216 Kit ELISA, FER1 Kit ELISA, FER1HCH Kit ELISA, Fer-L Kit ELISA, Fer1 Kit ELISA, Fer1HC Kit ELISA, FerHCH Kit ELISA, HCH Kit ELISA, T21 Kit ELISA, T9 Kit ELISA, fer1 Kit ELISA, fer1hch Kit ELISA, l(3)00451 Kit ELISA, l(3)j10B4 Kit ELISA, SFerH-1 Kit ELISA, SOF-35 Kit ELISA, SOF-5L Kit ELISA, NV13011 Kit ELISA, ACP1 Kit ELISA, F8A5.24 Kit ELISA, F8A5_24 Kit ELISA, Ferritin 1 heavy chain homologue Kit ELISA, ferritin light chain Kit ELISA, ferritin Kit ELISA, chromosome 26 open reading frame, human C10orf131 Kit ELISA, hyaluronan and proteoglycan link protein 2 Kit ELISA, NAD(P)-linked oxidoreductase superfamily protein Kit ELISA, Fer1HCH Kit ELISA, H-1 Kit ELISA, YPDSF_1340 Kit ELISA, ftnA Kit ELISA, Ft Kit ELISA, C26H10orf131 Kit ELISA, HAPLN2 Kit ELISA, AT1G60730 Kit ELISA

Sujet:

Gene Name: Ferritin

Pathways: Transition Metal Ion Homeostasis