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FTH1 Kit ELISA

FTH1 Reactivité: Humain Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
N° du produit ABIN5654118
  • Antigène Voir toutes FTH1 Kits ELISA
    FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))
    Reactivité
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Ferritin, Heavy Polypeptide (FTH). No significant cross-reactivity or interference between Ferritin, Heavy Polypeptide (FTH) and analogues was observed.
    Sensibilité
    12.9 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin, Heavy Polypeptide (FTH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferritin, Heavy Polypeptide (FTH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin, Heavy Polypeptide (FTH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferritin, Heavy Polypeptide (FTH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes FTH1 Kits ELISA
    FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))
    Autre désignation
    Ferritin, Heavy Polypeptide (FTH1 Produits)
    Synonymes
    FHC Kit ELISA, FTH Kit ELISA, FTHL6 Kit ELISA, PIG15 Kit ELISA, PLIF Kit ELISA, apoferritin Kit ELISA, ferritin Kit ELISA, fhc Kit ELISA, fth Kit ELISA, fth1 Kit ELISA, fthl6 Kit ELISA, ftn-2 Kit ELISA, pig15 Kit ELISA, plif Kit ELISA, fth1b Kit ELISA, Fth Kit ELISA, HFt Kit ELISA, MFH Kit ELISA, fb06g09 Kit ELISA, hm:zeh1145 Kit ELISA, wu:fb06g09 Kit ELISA, wu:fq18c10 Kit ELISA, zeh1145 Kit ELISA, ferritin heavy chain 1 Kit ELISA, ferritin, heavy polypeptide 1 L homeolog Kit ELISA, ferritin, heavy polypeptide 1 S homeolog Kit ELISA, ferritin heavy polypeptide 1 Kit ELISA, ferritin, heavy polypeptide 1a Kit ELISA, ferritin Kit ELISA, ferritin, heavy polypeptide 1 Kit ELISA, tudor domain containing 9 Kit ELISA, ferritin, heavy polypeptide 1 a Kit ELISA, FTH1 Kit ELISA, fth1.L Kit ELISA, fth1.S Kit ELISA, Fth1 Kit ELISA, fth1a Kit ELISA, EAMY_RS19690 Kit ELISA, fth1 Kit ELISA, TDRD9 Kit ELISA
    Sujet

    Gene Name: Ferritin, Heavy Polypeptide

    Gene Aliases: FTH1, FTHL6, PIG15, PLIF, Ferritin Heavy Chain, Apoferritin, Placenta Immunoregulatory Factor, Proliferation-Inducing Protein 15, Cell proliferation-inducing gene 15 protein

    ID gène
    2495
    UniProt
    P02794
    Pathways
    Transition Metal Ion Homeostasis
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