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FTL Kit ELISA

FTL Reactivité: Souris Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5654122
  • Antigène Voir toutes FTL Kits ELISA
    FTL (Ferritin, Light Polypeptide (FTL))
    Reactivité
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56 ng/mL - 100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Ferritin, Light Polypeptide (FTL). No significant cross-reactivity or interference between Ferritin, Light Polypeptide (FTL) and analogues was observed.
    Sensibilité
    0.67 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin, Light Polypeptide (FTL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferritin, Light Polypeptide (FTL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin, Light Polypeptide (FTL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferritin, Light Polypeptide (FTL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin, Light Polypeptide (FTL) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin, Light Polypeptide (FTL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes FTL Kits ELISA
    FTL (Ferritin, Light Polypeptide (FTL))
    Autre désignation
    Ferritin, Light Polypeptide (FTL Produits)
    Synonymes
    NBIA3 Kit ELISA, apoferritin Kit ELISA, ferritin Kit ELISA, fhc Kit ELISA, fth Kit ELISA, fth1 Kit ELISA, fthl6 Kit ELISA, ftn-2 Kit ELISA, pig15 Kit ELISA, plif Kit ELISA, FT Kit ELISA, Ftl Kit ELISA, Ftl-1 Kit ELISA, L-ferritin Kit ELISA, FTL Kit ELISA, Ftl1 Kit ELISA, FTL2 Kit ELISA, FTD Kit ELISA, ferritin light chain Kit ELISA, ferritin, heavy polypeptide 1 L homeolog Kit ELISA, ferritin light polypeptide 1 Kit ELISA, ferritin, light polypeptide Kit ELISA, ferritin light-chain Kit ELISA, ferritin light chain 1 Kit ELISA, FTL Kit ELISA, fth1.L Kit ELISA, Ftl1 Kit ELISA, CpipJ_CPIJ004069 Kit ELISA, Ftl Kit ELISA
    Sujet

    Gene Name: Ferritin, Light Polypeptide

    Gene Aliases: Ferritin Light Chain, Ferritin Light Polypeptide-Like 3, L Apoferritin, Ferritin L Subunit

    UniProt
    P29391
    Pathways
    Transition Metal Ion Homeostasis
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