FGG Kit ELISA

N° du produit ABIN5654156

Détail du produit FGG Kit ELISA

Antigène: FGG (Fibrinogen gamma Chain (FGG))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 ng/mL - 500 ng/mL

Seuil minimal de détection: 7.81 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Fibrinogen Gamma (FGg). No significant cross-reactivity or interference between Fibrinogen Gamma (FGg) and analogues was observed.

Sensibilité: 2.7 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Gamma (FGg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Gamma (FGg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Gamma (FGg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Gamma (FGg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Gamma (FGg) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Gamma (FGg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FGG (Fibrinogen gamma Chain (FGG))

Autre désignation: Fibrinogen Gamma (FGG Produits)

Synonymes: FXII Kit ELISA, HAF Kit ELISA, 3010002H13Rik Kit ELISA, AI256424 Kit ELISA, fibrinogen Kit ELISA, FGG Kit ELISA, LOC100220680 Kit ELISA, fb60h05 Kit ELISA, fb62e01 Kit ELISA, wu:fb60h05 Kit ELISA, wu:fb62e01 Kit ELISA, zgc:56023 Kit ELISA, fibrinogen gamma chain Kit ELISA, coagulation factor XII (Hageman factor) Kit ELISA, fibrinogen gamma chain L homeolog Kit ELISA, FGG Kit ELISA, F12 Kit ELISA, fgg Kit ELISA, Fgg Kit ELISA, fgg.L Kit ELISA, CpipJ_CPIJ006387 Kit ELISA, CpipJ_CPIJ010087 Kit ELISA

Sujet:

Gene Name: Fibrinogen Gamma

Gene Aliases: FG-G

ID gène: 24367

UniProt: P02680