FKBP1A Kit ELISA

N° du produit ABIN5654357

Détail du produit FKBP1A Kit ELISA

Antigène: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 1A (FKBP1A). No significant cross-reactivity or interference between FK506 Binding Protein 1A (FKBP1A) and analogues was observed.

Sensibilité: 11.8 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 1A (FKBP1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 1A (FKBP1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 1A (FKBP1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 1A (FKBP1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 1A (FKBP1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 1A (FKBP1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Autre désignation: FK506 Binding Protein 1A (FKBP1A Produits)

Synonymes: 12kDa Kit ELISA, FKBP12 Kit ELISA, FKBP12-T1 Kit ELISA, FKBP12-T2 Kit ELISA, Fkbp Kit ELISA, Fkbp1 Kit ELISA, mFKBP1 Kit ELISA, mFKBP12 Kit ELISA, FKBP-12 Kit ELISA, FKBP-1A Kit ELISA, FKBP1 Kit ELISA, Rotamase Kit ELISA, Fkbp12 Kit ELISA, ARABIDOPSIS THALIANA FK506-BINDING PROTEIN 12 Kit ELISA, ATFKBP12 Kit ELISA, FK506-binding protein 12 Kit ELISA, IMMUNOPHILIN Kit ELISA, MSJ1.19 Kit ELISA, MSJ1_19 Kit ELISA, PKC12 Kit ELISA, PKCI2 Kit ELISA, PPIASE Kit ELISA, FKBP1B Kit ELISA, Fkbp2 Kit ELISA, fj02g10 Kit ELISA, zgc:73373 Kit ELISA, wu:fj02g10 Kit ELISA, FKBP1A Kit ELISA, Bmfkbp13 Kit ELISA, zgc:103752 Kit ELISA, FK506 binding protein 1a Kit ELISA, FK506 binding protein 1A Kit ELISA, FK506-binding protein Kit ELISA, FK506-binding protein 12 Kit ELISA, FK506 binding protein 1Aa Kit ELISA, FK506 binding protein 1C Kit ELISA, FK506-binding protein 59 Kit ELISA, FK506 binding protein like Kit ELISA, FK506 binding protein 1A L homeolog Kit ELISA, FK506 binding protein 1Ab Kit ELISA, Fkbp1a Kit ELISA, FKBP1A Kit ELISA, Fkbp12 Kit ELISA, FKBP12 Kit ELISA, fkbp1aa Kit ELISA, FKBP1C Kit ELISA, EHI_180160 Kit ELISA, LOC5573443 Kit ELISA, Fkbp13 Kit ELISA, FKBPL Kit ELISA, fkbp1a.L Kit ELISA, fkbp1ab Kit ELISA

Sujet:

Gene Name: FK506 Binding Protein 1A

Gene Aliases: FKBP-12, FKBP1, FKBP12C, PKC12, PKCI2, Calstabin-1, Rotamase, 12 kDa FK506-binding protein, Peptidyl-prolyl cis-trans isomerase FKBP1A, Immunophilin FKBP12

ID gène: 2280

UniProt: P62942

Pathways: Negative Regulation of Transporter Activity, Methionine Biosynthetic Process