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FKBP1B Kit ELISA

FKBP1B Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5654358
  • Antigène Voir toutes FKBP1B Kits ELISA
    FKBP1B (FK506 Binding Protein 1B, 12.6 KDa (FKBP1B))
    Reactivité
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 1B (FKBP1B). No significant cross-reactivity or interference between FK506 Binding Protein 1B (FKBP1B) and analogues was observed.
    Sensibilité
    0.053 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 1B (FKBP1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 1B (FKBP1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 1B (FKBP1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 1B (FKBP1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 1B (FKBP1B) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 1B (FKBP1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes FKBP1B Kits ELISA
    FKBP1B (FK506 Binding Protein 1B, 12.6 KDa (FKBP1B))
    Autre désignation
    FK506 Binding Protein 1B (FKBP1B Produits)
    Synonymes
    FKBP12.6 Kit ELISA, MGC68829 Kit ELISA, FKBP1B Kit ELISA, DKFZp459K1429 Kit ELISA, 12.6kDa Kit ELISA, AW494148 Kit ELISA, calstabin2 Kit ELISA, FKBP1L Kit ELISA, OTK4 Kit ELISA, PKBP1L Kit ELISA, PPIase Kit ELISA, FK506 binding protein 1B Kit ELISA, FK506 binding protein 1B L homeolog Kit ELISA, FK506 binding protein 1b Kit ELISA, FKBP1B Kit ELISA, fkbp1b.L Kit ELISA, fkbp1b Kit ELISA, Fkbp1b Kit ELISA
    Sujet

    Gene Name: FK506 Binding Protein 1B

    Gene Aliases: FKBP12.6, FKBP1L, FKBP9, OTK4, PKBP1L, Rotamase, 12.6 kDa FK506-binding protein, Immunophilin FKBP12.6, Peptidyl-prolyl cis-trans isomerase FKBP1B

    ID gène
    2281
    UniProt
    P68106
    Pathways
    Hormone Transport, Negative Regulation of Hormone Secretion, Negative Regulation of Transporter Activity
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