FKBP4 Kit ELISA

N° du produit ABIN5654360

Détail du produit FKBP4 Kit ELISA

Antigène: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of FK506 Binding Protein 4 (FKBP4). No significant cross-reactivity or interference between FK506 Binding Protein 4 (FKBP4) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 4 (FKBP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FK506 Binding Protein 4 (FKBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FK506 Binding Protein 4 (FKBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FK506 Binding Protein 4 (FKBP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FK506 Binding Protein 4 (FKBP4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FK506 Binding Protein 4 (FKBP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Autre désignation: FK506 Binding Protein 4 (FKBP4 Produits)

Synonymes: HBI Kit ELISA, p52 Kit ELISA, hsp56 Kit ELISA, PPIase Kit ELISA, fkbp52 Kit ELISA, fkbp59 Kit ELISA, MGC81078 Kit ELISA, wu:fb34f09 Kit ELISA, 59kDa Kit ELISA, AL022792 Kit ELISA, AW208983 Kit ELISA, FKBP-4 Kit ELISA, FKBP-52 Kit ELISA, FKBP52 Kit ELISA, FKPB52 Kit ELISA, p59 Kit ELISA, FKBP51 Kit ELISA, FKBP59 Kit ELISA, Hsp56 Kit ELISA, P59 Kit ELISA, FK506 binding protein 4 Kit ELISA, FK506 binding protein 4 S homeolog Kit ELISA, FKBP4 Kit ELISA, fkbp4.S Kit ELISA, fkbp4 Kit ELISA, Fkbp4 Kit ELISA

Sujet:

Gene Name: FK506 Binding Protein 4

Gene Aliases: FKBP52, FKBP59, HBI, Hsp56, p52, Rotamase, 51 kDa FK506-binding protein, 59 kDa immunophilin, HSP-binding immunophilin, Peptidyl-prolyl cis-trans isomerase FKBP4

ID gène: 2288

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway