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FSH Kit ELISA

FSH Reactivité: Souris Colorimetric Competition ELISA 2.22 ng/mL - 180 ng/mL Plasma, Serum
N° du produit ABIN5654388
  • Antigène Voir toutes FSH Kits ELISA
    FSH (Follicle-Stimulating Hormone (FSH))
    Reactivité
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    2.22 ng/mL - 180 ng/mL
    Seuil minimal de détection
    2.22 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Follicle Stimulating Hormone (FSH). No significant cross-reactivity or interference between Follicle Stimulating Hormone (FSH) and analogues was observed.
    Sensibilité
    0.87 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Follicle Stimulating Hormone (FSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Follicle Stimulating Hormone (FSH) and unlabeled Follicle Stimulating Hormone (FSH) (Standards or samples) with the pre-coated antibody specific to Follicle Stimulating Hormone (FSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes FSH Kits ELISA
    FSH (Follicle-Stimulating Hormone (FSH))
    Autre désignation
    Follicle Stimulating Hormone (FSH Produits)
    Synonymes
    FSH Kit ELISA, Fshbeta Kit ELISA, follicle stimulating hormone beta Kit ELISA, Plasma FSH concentration Kit ELISA, Fshb Kit ELISA, FSH Kit ELISA
    Sujet

    Gene Name: Follicle Stimulating Hormone

    Pathways
    Peptide Hormone Metabolism, Chromatin Binding
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