Follistatin Kit ELISA

N° du produit ABIN5654395

Détail du produit Follistatin Kit ELISA

Antigène: Follistatin (FST)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 2.47 ng/mL - 200 ng/mL

Seuil minimal de détection: 2.47 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Follistatin (FS). No significant cross-reactivity or interference between Follistatin (FS) and analogues was observed.

Sensibilité: 0.97 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Follistatin (FS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Follistatin (FS) and unlabeled Follistatin (FS) (Standards or samples) with the pre-coated antibody specific to Follistatin (FS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follistatin (FS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Follistatin (FS) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin (FS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin (FS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Follistatin (FST)

Autre désignation: Follistatin (FST Produits)

Synonymes: CG12955 Kit ELISA, CG12956 Kit ELISA, CG33466 Kit ELISA, Dmel\\CG33466 Kit ELISA, Fol1 Kit ELISA, dFS Kit ELISA, dFol1 Kit ELISA, dfs Kit ELISA, fs Kit ELISA, FS Kit ELISA, foll Kit ELISA, xfs Kit ELISA, FST Kit ELISA, fst Kit ELISA, AL033346 Kit ELISA, FOL1 Kit ELISA, Fst-288 Kit ELISA, RATFOL1 Kit ELISA, cb446 Kit ELISA, fd07h10 Kit ELISA, fst1 Kit ELISA, wu:fd07h10 Kit ELISA, Follistatin Kit ELISA, follistatin Kit ELISA, follistatin L homeolog Kit ELISA, follistatin a Kit ELISA, Fs Kit ELISA, fst Kit ELISA, FST Kit ELISA, LOC100141662 Kit ELISA, fst.L Kit ELISA, Fst Kit ELISA, fsta Kit ELISA

Sujet:

Gene Name: Follistatin

Gene Aliases: FST, Activin-binding protein

Pathways: Negative Regulation of Hormone Secretion