GLB1 Kit ELISA

N° du produit ABIN5654470

Détail du produit GLB1 Kit ELISA

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125 pg/mL - 8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Galactosidase Beta (GLb). No significant cross-reactivity or interference between Galactosidase Beta (GLb) and analogues was observed.

Sensibilité: 54 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactosidase Beta (GLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactosidase Beta (GLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactosidase Beta (GLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galactosidase Beta (GLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galactosidase Beta (GLb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galactosidase Beta (GLb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: Galactosidase Beta (GLB1 Produits)

Synonymes: EBP Kit ELISA, ELNR1 Kit ELISA, MPS4B Kit ELISA, DER Kit ELISA, XR Kit ELISA, 0610038K04Rik Kit ELISA, 1810027P18Rik Kit ELISA, glb Kit ELISA, CG9092 Kit ELISA, Dmel\\CG9092 Kit ELISA, beta-GAL Kit ELISA, beta-Gal-1 Kit ELISA, beta-gal Kit ELISA, gal Kit ELISA, lacZ-1 Kit ELISA, AW125515 Kit ELISA, Bge Kit ELISA, Bgl Kit ELISA, Bgl-e Kit ELISA, Bgl-s Kit ELISA, Bgl-t Kit ELISA, Bgs Kit ELISA, Bgt Kit ELISA, C130097A14Rik Kit ELISA, BGAL Kit ELISA, zgc:110823 Kit ELISA, ECK0341 Kit ELISA, JW0335 Kit ELISA, GLB Kit ELISA, galactosidase beta 1 Kit ELISA, dicarbonyl and L-xylulose reductase Kit ELISA, dicarbonyl L-xylulose reductase Kit ELISA, beta-D-galactosidase Kit ELISA, beta-galactosidase Kit ELISA, beta galactosidase Kit ELISA, galactosidase, beta 1 Kit ELISA, beta-galactosidase-like Kit ELISA, GLB1 Kit ELISA, DCXR Kit ELISA, Dcxr Kit ELISA, lacZ Kit ELISA, SSP_RS00525 Kit ELISA, lacH Kit ELISA, ECL_03691 Kit ELISA, Gal Kit ELISA, Glb1 Kit ELISA, glb1 Kit ELISA, VV2_1327 Kit ELISA, LOC100056371 Kit ELISA

Sujet:

Gene Name: Galactosidase Beta

Gene Aliases: GL-B, b-GAL, bGAL, ELNR1, EBP, GLB1, Lactase, Elastin Receptor 1,67 kDa, Elastin Receptor 1(67kD), Acid Beta-Galactosidase

ID gène: 2720

UniProt: P16278

Pathways: Glycosaminoglycan Metabolic Process