GDI1 Kit ELISA

N° du produit ABIN5654540

Détail du produit GDI1 Kit ELISA

Antigène: GDI1 (GDP Dissociation Inhibitor 1 (GDI1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of GDP Dissociation Inhibitor 1 (GDI1). No significant cross-reactivity or interference between GDP Dissociation Inhibitor 1 (GDI1) and analogues was observed.

Sensibilité: 0.052 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDP Dissociation Inhibitor 1 (GDI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GDP Dissociation Inhibitor 1 (GDI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GDP Dissociation Inhibitor 1 (GDI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GDP Dissociation Inhibitor 1 (GDI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDP Dissociation Inhibitor 1 (GDI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDP Dissociation Inhibitor 1 (GDI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GDI1 (GDP Dissociation Inhibitor 1 (GDI1))

Autre désignation: GDP Dissociation Inhibitor 1 (GDI1 Produits)

Synonymes: 1A Kit ELISA, GDIL Kit ELISA, MRX41 Kit ELISA, MRX48 Kit ELISA, OPHN2 Kit ELISA, RABGD1A Kit ELISA, RABGDIA Kit ELISA, XAP-4 Kit ELISA, GDIA Kit ELISA, GDIalpha Kit ELISA, GDI-1 Kit ELISA, zgc:110600 Kit ELISA, QccE-14779 Kit ELISA, Rab GDI alpha Kit ELISA, GDP dissociation inhibitor 1 Kit ELISA, GDP dissociation inhibitor 1 S homeolog Kit ELISA, guanosine diphosphate (GDP) dissociation inhibitor 1 Kit ELISA, GDI1 Kit ELISA, gdi1.S Kit ELISA, Gdi1 Kit ELISA, gdi1 Kit ELISA

Sujet:

Gene Name: GDP Dissociation Inhibitor 1

Gene Aliases: GDIL, MRX41, MRX48, OPHN2, RABGD1A, RABGDIA, XAP-4, Guanine Nucleotide Dissociation Inhibitor, Rab GDP Dissociation Inhibitor Alpha 1, Oligophrenin-2

ID gène: 2664

UniProt: P31150