Glucose-6-Phosphate Dehydrogenase Kit ELISA

N° du produit ABIN5654613

Détail du produit Glucose-6-Phosphate Dehydrogenase Kit ELISA

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glucose 6 Phosphate Dehydrogenase (G6PD). No significant cross-reactivity or interference between Glucose 6 Phosphate Dehydrogenase (G6PD) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Dehydrogenase (G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Dehydrogenase (G6PD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose 6 Phosphate Dehydrogenase (G6PD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose 6 Phosphate Dehydrogenase (G6PD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Autre désignation: Glucose 6 Phosphate Dehydrogenase (G6PD Produits)

Synonymes: G6PD1 Kit ELISA, g6pd Kit ELISA, G6pd Kit ELISA, G6pdx Kit ELISA, g6pdh Kit ELISA, g6pd2 Kit ELISA, fj78b06 Kit ELISA, wu:fj78b06 Kit ELISA, si:dkey-90a13.8 Kit ELISA, BA3433 Kit ELISA, CORTRD1 Kit ELISA, G6PDH Kit ELISA, GDH Kit ELISA, G28A Kit ELISA, G6PD Kit ELISA, Gpdx Kit ELISA, glucose-6-phosphate dehydrogenase Kit ELISA, glucose-6-phosphate 1-dehydrogenase Kit ELISA, glucose-6-phosphate 1-dehydrogenase (G6PD) Kit ELISA, glucose-6-P dehydrogenase Kit ELISA, glucose-6-phosphate dehydrogenase L homeolog Kit ELISA, glucose-6-phosphate 1-dehydrogenase Zwf Kit ELISA, hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Kit ELISA, glucose-6-phosphate dehydrogenase X-linked Kit ELISA, G6PD Kit ELISA, g6pd Kit ELISA, g6pD Kit ELISA, zwf Kit ELISA, LOC100120232 Kit ELISA, LACBIDRAFT_188936 Kit ELISA, G6pd Kit ELISA, g6pd.L Kit ELISA, CNG03280 Kit ELISA, Tb10.70.5200 Kit ELISA, H6PD Kit ELISA, G6pdx Kit ELISA, H6pd Kit ELISA

Sujet:

Gene Name: Glucose 6 Phosphate Dehydrogenase

Gene Aliases: Glucose-6-phosphate 1-dehydrogenase

ID gène: 24377

UniProt: P05370

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones