GCLC Kit ELISA

N° du produit ABIN5654642

Détail du produit GCLC Kit ELISA

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutamate Cysteine Ligase, Catalytic (GCLC). No significant cross-reactivity or interference between Glutamate Cysteine Ligase, Catalytic (GCLC) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, Catalytic (GCLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, Catalytic (GCLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Cysteine Ligase, Catalytic (GCLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Cysteine Ligase, Catalytic (GCLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Catalytic (GCLC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Catalytic (GCLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Autre désignation: Glutamate Cysteine Ligase, Catalytic (GCLC Produits)

Synonymes: GCLC Kit ELISA, gclc Kit ELISA, MGC146287 Kit ELISA, 2259 Kit ELISA, CG2259 Kit ELISA, DmGCLC Kit ELISA, DmGCS Kit ELISA, DmGCSh Kit ELISA, Dmel\\CG2259 Kit ELISA, GCL Kit ELISA, GCLc Kit ELISA, GCSh Kit ELISA, Gcs Kit ELISA, Gcsh Kit ELISA, DDBDRAFT_0186120 Kit ELISA, DDBDRAFT_0231403 Kit ELISA, DDB_0186120 Kit ELISA, DDB_0231403 Kit ELISA, GCS Kit ELISA, GLCL Kit ELISA, GLCLC Kit ELISA, D9Wsu168e Kit ELISA, GLCL-H Kit ELISA, Ggcs-hs Kit ELISA, Glclc Kit ELISA, cb1049 Kit ELISA, fe36e11 Kit ELISA, wu:fe36e11 Kit ELISA, glutamate-cysteine ligase catalytic subunit Kit ELISA, glutamate-cysteine ligase, catalytic subunit Kit ELISA, Glutamate-cysteine ligase catalytic subunit Kit ELISA, gamma glutamylcysteine synthetase Kit ELISA, glutamate-cysteine ligase, catalytic subunit L homeolog Kit ELISA, glutamate-cysteine ligase, catalytic subunit S homeolog Kit ELISA, gamma-glutamylcysteine synthetase Kit ELISA, GCLC Kit ELISA, gclc Kit ELISA, Gclc Kit ELISA, LbGCS Kit ELISA, gcsA Kit ELISA, gclc.L Kit ELISA, gclc.S Kit ELISA, GSH1 Kit ELISA

Sujet:

Gene Name: Glutamate Cysteine Ligase, Catalytic

Gene Aliases: GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS

ID gène: 25283

UniProt: P19468

Pathways: Cell RedoxHomeostasis