Glutamate Receptor 1 Kit ELISA

N° du produit ABIN5654657

Détail du produit Glutamate Receptor 1 Kit ELISA

Antigène: Glutamate Receptor 1 (GLUR1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1). No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Glutamate Receptor 1 (GLUR1)

Autre désignation: Glutamate Receptor, Ionotropic, AMPA 1 (GLUR1 Produits)

Synonymes: GLUH1 Kit ELISA, GLUR1 Kit ELISA, GLURA Kit ELISA, GluA1 Kit ELISA, HBGR1 Kit ELISA, gluR-A Kit ELISA, 2900051M01Rik Kit ELISA, Glr-1 Kit ELISA, Glr1 Kit ELISA, GluR-A Kit ELISA, GluRA Kit ELISA, Glur-1 Kit ELISA, Glur1 Kit ELISA, HIPA1 Kit ELISA, gluR-K1 Kit ELISA, GRIA1 Kit ELISA, glur1 Kit ELISA, Gria1 Kit ELISA, glur1b Kit ELISA, gria1.2 Kit ELISA, sb:eu293 Kit ELISA, ZfGluR1a Kit ELISA, glur1a Kit ELISA, gria1.1 Kit ELISA, zfGRIA1[a] Kit ELISA, glutamate ionotropic receptor AMPA type subunit 1 Kit ELISA, glutamate receptor, ionotropic, AMPA1 (alpha 1) Kit ELISA, glutamate receptor, ionotropic, AMPA 1 Kit ELISA, glutamate receptor, ionotropic, AMPA 1 L homeolog Kit ELISA, GluR1 protein Kit ELISA, Glutamate receptor 1 Kit ELISA, glutamate receptor, ionotropic, AMPA 1b Kit ELISA, glutamate receptor, ionotropic, AMPA 1a Kit ELISA, GRIA1 Kit ELISA, Gria1 Kit ELISA, gria1 Kit ELISA, gria1.L Kit ELISA, glur1 Kit ELISA, glr-1 Kit ELISA, gria1b Kit ELISA, gria1a Kit ELISA

Sujet:

Gene Name: Glutamate Receptor, Ionotropic, AMPA 1

Gene Aliases: GLUH1, GLUR1, GLURA, HBGR1, GluR-K1, AMPA-selective glutamate receptor 1

Pathways: Signalisation PI3K-Akt