Glutathione Reductase Kit ELISA

N° du produit ABIN5654702

Détail du produit Glutathione Reductase Kit ELISA

Antigène: Glutathione Reductase (GSR)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutathione Reductase (GR). No significant cross-reactivity or interference between Glutathione Reductase (GR) and analogues was observed.

Sensibilité: 37 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Reductase (GR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Reductase (GR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Reductase (GR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Reductase (GR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Reductase (GR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Reductase (GR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Glutathione Reductase (GSR)

Autre désignation: Glutathione Reductase (GSR Produits)

Synonymes: GR Kit ELISA, ATGR2 Kit ELISA, EMB2360 Kit ELISA, glutathione reductase Kit ELISA, AI325518 Kit ELISA, D8Ertd238e Kit ELISA, Gr-1 Kit ELISA, Gr1 Kit ELISA, DDBDRAFT_0168952 Kit ELISA, DDBDRAFT_0231410 Kit ELISA, DDB_0168952 Kit ELISA, DDB_0231410 Kit ELISA, 2151 Kit ELISA, CG2151 Kit ELISA, DTR Kit ELISA, Dm-TrxR Kit ELISA, DmTR Kit ELISA, DmTrx Kit ELISA, DmTrxR Kit ELISA, DmTrxR-1 Kit ELISA, Dmel\\CG2151 Kit ELISA, Gr Kit ELISA, Trx Kit ELISA, TrxR Kit ELISA, TrxR-1 Kit ELISA, Trxr1 Kit ELISA, anon-WO03040301.185 Kit ELISA, anon-WO03040301.187 Kit ELISA, dTrxR Kit ELISA, dmtrxr-1 Kit ELISA, gr Kit ELISA, l(1)G0154 Kit ELISA, l(1)G0379 Kit ELISA, l(1)G0477 Kit ELISA, l(1)G0481 Kit ELISA, trxr-1 Kit ELISA, gor1 Kit ELISA, glutathione reductase, gro-2 Kit ELISA, glutathione reductase Kit ELISA, glutathione-disulfide reductase Kit ELISA, GR; GRase Kit ELISA, mitochondrial glutathione reductase Pgr1 Kit ELISA, Glutathione reductase (GR) (GRase) Kit ELISA, Thioredoxin reductase-1 Kit ELISA, glutathione-disulfide reductase family protein Kit ELISA, glutathione reductase 1 Kit ELISA, GR Kit ELISA, gor Kit ELISA, GSR Kit ELISA, GSR1 Kit ELISA, Gsr_2 Kit ELISA, Gsr Kit ELISA, GSHR1 Kit ELISA, gsr Kit ELISA, LbGR Kit ELISA, pgr1 Kit ELISA, GSHR2 Kit ELISA, GLR2 Kit ELISA, Bcen_2392 Kit ELISA, RPE_1989 Kit ELISA, HCAG_02219 Kit ELISA, SJAG_01162 Kit ELISA, Trxr-1 Kit ELISA, PF14_0192 Kit ELISA, POPTR_0001s14480g Kit ELISA, gsr1 Kit ELISA

Sujet:

Gene Name: Glutathione Reductase

Gene Aliases: GSR, GLUR, GRD1, Glutathione reductase, mitochondrial

ID gène: 14782

UniProt: P47791

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis