GAPDH Kit ELISA

N° du produit ABIN5654735

Détail du produit GAPDH Kit ELISA

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). No significant cross-reactivity or interference between Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH Produits)

Synonymes: G3PD Kit ELISA, GAPD Kit ELISA, Gapd Kit ELISA, BEST:GH12586 Kit ELISA, CG12055 Kit ELISA, Dmel\\CG12055 Kit ELISA, GA3PDH Kit ELISA, GADPH Kit ELISA, GAP Kit ELISA, GAPDH Kit ELISA, GAPDH I Kit ELISA, GAPDH-1 Kit ELISA, GAPDH1 Kit ELISA, GAPDHI Kit ELISA, Gapdh Kit ELISA, Gapdh-1 Kit ELISA, Gapdh43E Kit ELISA, gadph Kit ELISA, gapdh Kit ELISA, gapdh-1 Kit ELISA, gh12586 Kit ELISA, cb609 Kit ELISA, gapd Kit ELISA, mg:bb02e05 Kit ELISA, wu:fb33a10 Kit ELISA, wu:ft80f05 Kit ELISA, KNC-NDS6 Kit ELISA, g3pd Kit ELISA, G3PDH Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase Kit ELISA, Glyceraldehyde 3 phosphate dehydrogenase 1 Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase S homeolog Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase, type I Kit ELISA, olfactory receptor 8K3 Kit ELISA, GAPDH Kit ELISA, Gapdh Kit ELISA, Gapdh1 Kit ELISA, gapdh Kit ELISA, gapdh.S Kit ELISA, gapDH Kit ELISA, LOC100404960 Kit ELISA, LOC614985 Kit ELISA

Sujet:

Gene Name: Glyceraldehyde-3-Phosphate Dehydrogenase

Gene Aliases: G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH

ID gène: 100042025

UniProt: P16858