GLDC Kit ELISA

N° du produit ABIN5654739

Détail du produit GLDC Kit ELISA

Antigène: GLDC (Glycine Dehydrogenase (GLDC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glycine Dehydrogenase (GLDC). No significant cross-reactivity or interference between Glycine Dehydrogenase (GLDC) and analogues was observed.

Sensibilité: 0.132 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycine Dehydrogenase (GLDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycine Dehydrogenase (GLDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycine Dehydrogenase (GLDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GLDC (Glycine Dehydrogenase (GLDC))

Autre désignation: Glycine Dehydrogenase (GLDC Produits)

Synonymes: PSPTO1276 Kit ELISA, Tb07.43M14.350 Kit ELISA, DDBDRAFT_0219205 Kit ELISA, DDBDRAFT_0231130 Kit ELISA, DDB_0219205 Kit ELISA, DDB_0231130 Kit ELISA, D030049L12Rik Kit ELISA, D19Wsu57e Kit ELISA, GCE Kit ELISA, GCSP Kit ELISA, HYGN1 Kit ELISA, fb23b05 Kit ELISA, wu:fb23b05 Kit ELISA, zgc:66198 Kit ELISA, gldc Kit ELISA, glycine dehydrogenase (decarboxylating) GcvP Kit ELISA, glycine dehydrogenase Kit ELISA, glycine cleavage system P-protein Kit ELISA, glycine decarboxylase Kit ELISA, glycine dehydrogenase (decarboxylating) Kit ELISA, glycine decarboxylase S homeolog Kit ELISA, gcvP Kit ELISA, Tb927.7.1910 Kit ELISA, Gldc Kit ELISA, GLDC Kit ELISA, gldc Kit ELISA, gldc.S Kit ELISA

Sujet:

Gene Name: Glycine Dehydrogenase

Gene Aliases: GCSP, NKH, Decarboxylating, Glycine Decarboxylase, Glycine Cleavage System Protein P, Glycine dehydrogenase (aminomethyl-transferring)

ID gène: 2731

UniProt: P23378