GARS Kit ELISA
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- Antigène Voir toutes GARS Kits ELISA
- GARS (Glycyl-tRNA Synthetase (GARS))
- Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.62 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.62 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Glycyl tRNA Synthetase (GARS). No significant cross-reactivity or interference between Glycyl tRNA Synthetase (GARS) and analogues was observed.
- Sensibilité
- 6.6 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycyl tRNA Synthetase (GARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycyl tRNA Synthetase (GARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycyl tRNA Synthetase (GARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycyl tRNA Synthetase (GARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycyl tRNA Synthetase (GARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycyl tRNA Synthetase (GARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes GARS Kits ELISA
- GARS (Glycyl-tRNA Synthetase (GARS))
- Autre désignation
- Glycyl tRNA Synthetase (GARS Produits)
- Synonymes
- CMT2D Kit ELISA, DSMAV Kit ELISA, GlyRS Kit ELISA, HMN5 Kit ELISA, SMAD1 Kit ELISA, Aat-gly Kit ELISA, CG6778 Kit ELISA, Dmel\\CG6778 Kit ELISA, GRS Kit ELISA, gars Kit ELISA, team Kit ELISA, RGD1559871 Kit ELISA, GENA202 Kit ELISA, Gena201 Kit ELISA, Nmf249 Kit ELISA, Sgrp23 Kit ELISA, GB13467 Kit ELISA, MGC79495 Kit ELISA, fb02f03 Kit ELISA, si:dkey-276i5.1 Kit ELISA, wu:fb02f03 Kit ELISA, NI36_08435 Kit ELISA, glycyl-tRNA synthetase Kit ELISA, Glycyl-tRNA synthetase Kit ELISA, glycine--tRNA ligase Kit ELISA, glycyl-tRNA synthetase L homeolog Kit ELISA, Glycine--tRNA ligase Kit ELISA, GARS Kit ELISA, GlyRS Kit ELISA, Gars Kit ELISA, LOC408392 Kit ELISA, gars.L Kit ELISA, gars Kit ELISA, LOC692991 Kit ELISA, glyQ Kit ELISA, TTHA0543 Kit ELISA, NI36_RS08405 Kit ELISA
- Sujet
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Gene Name: Glycyl tRNA Synthetase
Gene Aliases: CMT2D, CMT2-D, DSMAV, GlyRS, SMAD1, EJ, Charcot-Marie-Tooth Neuropathy 2D, Glycine tRNA Ligase, Diadenosine tetraphosphate synthetase, AP-4-A synthetase
- ID gène
- 2617
- UniProt
- P41250
- Pathways
- Ribonucleoside Biosynthetic Process
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