GOLM1 Kit ELISA

N° du produit ABIN5654787

Détail du produit GOLM1 Kit ELISA

Antigène: GOLM1 (Golgi Membrane Protein 1 (GOLM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Golgi Protein 73 (GP73). No significant cross-reactivity or interference between Golgi Protein 73 (GP73) and analogues was observed.

Sensibilité: 0.229 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi Protein 73 (GP73). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Golgi Protein 73 (GP73). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Golgi Protein 73 (GP73), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Golgi Protein 73 (GP73) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Golgi Protein 73 (GP73) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Golgi Protein 73 (GP73) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GOLM1 (Golgi Membrane Protein 1 (GOLM1))

Autre désignation: Golgi Protein 73 (GOLM1 Produits)

Synonymes: C9orf155 Kit ELISA, GOLPH2 Kit ELISA, GP73 Kit ELISA, PSEC0257 Kit ELISA, bA379P1.3 Kit ELISA, 2310001L02Rik Kit ELISA, AW125446 Kit ELISA, D030064E01Rik Kit ELISA, Golph2 Kit ELISA, GOLM1 Kit ELISA, golph2 Kit ELISA, gp73 Kit ELISA, golgi membrane protein 1 Kit ELISA, SPATA31 subfamily D, member 3 Kit ELISA, Golgi membrane protein 1 Kit ELISA, golgi membrane protein 1 L homeolog Kit ELISA, GOLM1 Kit ELISA, Golm1 Kit ELISA, Spata31d3 Kit ELISA, golm1 Kit ELISA, LOC100400549 Kit ELISA, golm1.L Kit ELISA

Sujet:

Gene Name: Golgi Protein 73

Gene Aliases: GOLM1, GOLPH2, C9orf155, Golgi Membrane Protein 1, Golgi Phosphoprotein 2

ID gène: 51280

UniProt: Q8NBJ4