GDA Kit ELISA
-
- Antigène Voir toutes GDA Kits ELISA
- GDA (Guanine Deaminase (GDA))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Guanine Deaminase (GDA). No significant cross-reactivity or interference between Guanine Deaminase (GDA) and analogues was observed.
- Sensibilité
- 0.129 ng/mL
- Featured
- Discover our best selling GDA Kit ELISA
- Top Product
- Discover our top product GDA Kit ELISA
-
-
- Commentaires
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanine Deaminase (GDA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanine Deaminase (GDA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanine Deaminase (GDA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guanine Deaminase (GDA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Guanine Deaminase (GDA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Guanine Deaminase (GDA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
-
- Antigène Voir toutes GDA Kits ELISA
- GDA (Guanine Deaminase (GDA))
- Autre désignation
- Guanine Deaminase (GDA Produits)
- Synonymes
- CYPIN Kit ELISA, GUANASE Kit ELISA, NEDASIN Kit ELISA, AU015411 Kit ELISA, AW047581 Kit ELISA, Cypin Kit ELISA, MGC81835 Kit ELISA, zgc:112282 Kit ELISA, AGR_C_4212 Kit ELISA, DDBDRAFT_0169373 Kit ELISA, DDBDRAFT_0230211 Kit ELISA, DDB_0169373 Kit ELISA, DDB_0230211 Kit ELISA, guanine deaminase Kit ELISA, guanine deaminase L homeolog Kit ELISA, short-chain dehydrogenase/reductase sdr Kit ELISA, GDA Kit ELISA, Gda Kit ELISA, gda.L Kit ELISA, gda Kit ELISA, SPO2956 Kit ELISA, Gdia_3192 Kit ELISA, Mchl_2046 Kit ELISA, Mnod_4655 Kit ELISA, guaD Kit ELISA, Dd586_1771 Kit ELISA, Kvar_2512 Kit ELISA, Mrub_1462 Kit ELISA, BC1002_2263 Kit ELISA, Cseg_1079 Kit ELISA, Mesil_1795 Kit ELISA, Trad_2850 Kit ELISA, Mesop_0346 Kit ELISA, gDA Kit ELISA, ML5_2802 Kit ELISA
- Sujet
-
Gene Name: Guanine Deaminase
Gene Aliases: CYPIN, Guanase, Guanine aminase, Guanine aminohydrolase, p51-nedasin
- ID gène
- 9615
- UniProt
- Q9Y2T3
-