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Haptoglobin Kit ELISA

HP Reactivité: Souris Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum
N° du produit ABIN5654937
  • Antigène Voir toutes Haptoglobin (HP) Kits ELISA
    Haptoglobin (HP)
    Reactivité
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    • 4
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    • 3
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    • 2
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    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 ng/mL - 2000 ng/mL
    Seuil minimal de détection
    31.25 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Haptoglobin (Hpt). No significant cross-reactivity or interference between Haptoglobin (Hpt) and analogues was observed.
    Sensibilité
    13.5 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Haptoglobin (Hpt). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Haptoglobin (Hpt). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Haptoglobin (Hpt), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Haptoglobin (Hpt) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Haptoglobin (Hpt) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Haptoglobin (Hpt) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Haptoglobin (HP) Kits ELISA
    Haptoglobin (HP)
    Autre désignation
    Haptoglobin (HP Produits)
    Synonymes
    HP Kit ELISA, wu:fb64e01 Kit ELISA, BP Kit ELISA, HP2ALPHA2 Kit ELISA, HPA1S Kit ELISA, HP-1 Kit ELISA, HPR Kit ELISA, Zonulin Kit ELISA, haptoglobin Kit ELISA, haptoglobin-like Kit ELISA, HP Kit ELISA, hp Kit ELISA, Hp Kit ELISA, LOC479668 Kit ELISA, LOC101102413 Kit ELISA
    Sujet

    Gene Name: Haptoglobin

    Gene Aliases: HP, Hp2-Alpha, Alpha-2-Macroglobulin, Zonulin

    ID gène
    15439
    UniProt
    Q61646
    Pathways
    Transition Metal Ion Homeostasis
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