HSP90 Kit ELISA

N° du produit ABIN5654978

Détail du produit HSP90 Kit ELISA

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90 (HSP90). No significant cross-reactivity or interference between Heat Shock Protein 90 (HSP90) and analogues was observed.

Sensibilité: 1.28 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90 (HSP90). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90 (HSP90). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90 (HSP90), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90 (HSP90) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90 (HSP90) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90 (HSP90) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Autre désignation: Heat Shock Protein 90 (HSP90 Produits)

Synonymes: EL52 Kit ELISA, HSP86 Kit ELISA, HSP89A Kit ELISA, HSP90A Kit ELISA, HSP90N Kit ELISA, HSPC1 Kit ELISA, HSPCA Kit ELISA, HSPCAL1 Kit ELISA, HSPCAL4 Kit ELISA, HSPN Kit ELISA, Hsp89 Kit ELISA, Hsp90 Kit ELISA, LAP2 Kit ELISA, git10 Kit ELISA, swo1 Kit ELISA, HSP90 Kit ELISA, htpG Kit ELISA, SCBAC25F8.08 Kit ELISA, 23.m06066 Kit ELISA, 17.m07646 Kit ELISA, HSP90-1 Kit ELISA, 143198_at Kit ELISA, 83 Kit ELISA, 83K HSP Kit ELISA, DMHSP82 Kit ELISA, E(sev)3A Kit ELISA, E(sina)2 Kit ELISA, HSP82 Kit ELISA, HSP83 Kit ELISA, ORF1 Kit ELISA, Su(Raf)3A Kit ELISA, anon-EST:Liang-2.53 Kit ELISA, anon-WO0068693 Kit ELISA, anon-WO0140519.209 Kit ELISA, clone 2.53 Kit ELISA, en(lz)3C/4C Kit ELISA, hsp84 Kit ELISA, l(3)j5C2 Kit ELISA, ms(3)08445 Kit ELISA, stc Kit ELISA, DmelCG1242 Kit ELISA, CG1242 Kit ELISA, Hsp86 Kit ELISA, Hspca Kit ELISA, 86kDa Kit ELISA, 89kDa Kit ELISA, AL024080 Kit ELISA, AL024147 Kit ELISA, Hsp86-1 Kit ELISA, hsp4 Kit ELISA, hsp86 Kit ELISA, hsp89 Kit ELISA, hsp90 Kit ELISA, hsp90a Kit ELISA, hspc1 Kit ELISA, hspca Kit ELISA, hspn Kit ELISA, lap2 Kit ELISA, Hsp90alpha Kit ELISA, heat shock protein 90 alpha family class A member 1 Kit ELISA, heat shock protein Hsp90 Kit ELISA, Hsp90 chaperone Kit ELISA, Heat shock protein 90 Kit ELISA, heat shock protein 90 Kit ELISA, chaperone protein HtpG Kit ELISA, molecular chaperone HtpG Kit ELISA, Heat Shock Protein 90 Kit ELISA, uncharacterized LOC100384473 Kit ELISA, Heat shock protein 83 Kit ELISA, heat shock protein 90, alpha (cytosolic), class A member 1 Kit ELISA, heat shock protein 90 alpha family class B member 1 Kit ELISA, heat shock protein 90kDa alpha family class A member 1 L homeolog Kit ELISA, heat shock protein HSP 90-alpha Kit ELISA, HSP90AA1 Kit ELISA, hsp90 Kit ELISA, HSP90 Kit ELISA, daf-21 Kit ELISA, htpG Kit ELISA, SCO7516 Kit ELISA, MAP_RS10510 Kit ELISA, TP04_0646 Kit ELISA, TP01_0934 Kit ELISA, APH_RS03525 Kit ELISA, GbCGDNIH1_0315 Kit ELISA, MAV_2118 Kit ELISA, HSP90C Kit ELISA, BBOV_IV008400 Kit ELISA, BBOV_III007380 Kit ELISA, ACICU_00312 Kit ELISA, ECL_01244 Kit ELISA, YE105_C1172 Kit ELISA, pco153543(105) Kit ELISA, Hsp83 Kit ELISA, Hsp90aa1 Kit ELISA, HSP90AB1 Kit ELISA, hsp90aa1.1.L Kit ELISA, LOC108698781 Kit ELISA

Sujet:

Gene Name: Heat Shock Protein 90

Gene Aliases: HtpG, Hsp-90 NTD, N-Terminal Domain

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals