HBA1 Kit ELISA

N° du produit ABIN5655027

Détail du produit HBA1 Kit ELISA

Antigène: HBA1 (Hemoglobin, alpha 1 (HBA1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hemoglobin Alpha 1 (HBa1). No significant cross-reactivity or interference between Hemoglobin Alpha 1 (HBa1) and analogues was observed.

Sensibilité: 0.63 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin Alpha 1 (HBa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin Alpha 1 (HBa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin Alpha 1 (HBa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Alpha 1 (HBa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin Alpha 1 (HBa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin Alpha 1 (HBa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HBA1 (Hemoglobin, alpha 1 (HBA1))

Autre désignation: Hemoglobin Alpha 1 (HBA1 Produits)

Synonymes: HBA1 Kit ELISA, HBAM Kit ELISA, Hba-a1 Kit ELISA, Hba-a2 Kit ELISA, cd31 Kit ELISA, hba-a Kit ELISA, hba2 Kit ELISA, HBH Kit ELISA, HBA2 Kit ELISA, alpha globin Kit ELISA, CD31 Kit ELISA, Hba Kit ELISA, Hba1 Kit ELISA, Hbat1 Kit ELISA, AA1 Kit ELISA, dZ118J2.2 Kit ELISA, HBAI Kit ELISA, HBAII Kit ELISA, hemoglobin, alpha 1 Kit ELISA, hemoglobin subunit alpha 1 Kit ELISA, hemoglobin subunit alpha 1 S homeolog Kit ELISA, hemoglobin subunit alpha 2 Kit ELISA, hemoglobin, alpha 2 Kit ELISA, hemoglobin subunit alpha-like Kit ELISA, hemoglobin alpha, adult chain 1 Kit ELISA, alpha-hemoglobin Kit ELISA, hemoglobin subunit alpha Kit ELISA, hemoglobin, alpha adult 1 Kit ELISA, hemoglobin subunit alpha-2-like Kit ELISA, HBA Kit ELISA, Hba1 Kit ELISA, hba1 Kit ELISA, hba1.S Kit ELISA, HBA1 Kit ELISA, HBA2 Kit ELISA, LOC100737768 Kit ELISA, Hba-a1 Kit ELISA, LOC100472013 Kit ELISA, LOC100087130 Kit ELISA, hbaa1 Kit ELISA, LOC102168959 Kit ELISA

Sujet:

Gene Name: Hemoglobin Alpha 1

Gene Aliases: Alpha-globin

UniProt: P69905