HNF1A Kit CLIA

N° du produit ABIN5655087

Détail du produit HNF1A Kit CLIA

Antigène: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Alpha (HNF1a). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Alpha (HNF1a) and analogues was observed.

Sensibilité: 5.75 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 1 Alpha (HNF1a) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HNF1A (HNF1 Homeobox A (HNF1A))

Autre désignation: Hepatocyte Nuclear Factor 1 Alpha (HNF1A Produits)

Synonymes: HNF-1A Kit CLIA, HNF1 Kit CLIA, IDDM20 Kit CLIA, LFB1 Kit CLIA, MODY3 Kit CLIA, TCF-1 Kit CLIA, TCF1 Kit CLIA, HNF-1a Kit CLIA, AI323641 Kit CLIA, HNF1-alpha Kit CLIA, HNF1[a] Kit CLIA, Hnf-1 Kit CLIA, Hnf1alpha Kit CLIA, Tcf-1 Kit CLIA, Tcf1 Kit CLIA, HNF-1-alpha Kit CLIA, HNF-1alpha Kit CLIA, LF-B1 Kit CLIA, Lfb1 Kit CLIA, HNF-1 Kit CLIA, HNF1A Kit CLIA, tcf1 Kit CLIA, FB1b Kit CLIA, HNF1b Kit CLIA, XLFB1b1 Kit CLIA, hnf1-B Kit CLIA, lfb1b Kit CLIA, mody3 Kit CLIA, tcf1-B Kit CLIA, tcfb-A Kit CLIA, HNF-1-alpha-A Kit CLIA, HNF-1A-A Kit CLIA, LFB1a1 Kit CLIA, TCF-1-A Kit CLIA, XLFB1 Kit CLIA, XLFB1b Kit CLIA, hnf1-a Kit CLIA, hnf1a Kit CLIA, hnf1a-a Kit CLIA, hnf1a-b Kit CLIA, hnf1a.L Kit CLIA, tcf1-A Kit CLIA, xx:af244934-22s Kit CLIA, HNF1 homeobox A Kit CLIA, HNF1 homeobox A L homeolog Kit CLIA, HNF1 homeobox A S homeolog Kit CLIA, HNF1 homeobox a Kit CLIA, HNF1A Kit CLIA, Hnf1a Kit CLIA, hnf1a Kit CLIA, hnf1a.L Kit CLIA, hnf1a.S Kit CLIA

Sujet:

Gene Name: Hepatocyte Nuclear Factor 1 Alpha

Gene Aliases: TCF1, HNF1, LFB1, LF-B1, MODY3, Transcription Factor 1,Hepatic, Hepatic Nuclear Factor, Albumin Proximal Factor, Liver-specific transcription factor LF-B1

Pathways: Hormone Transport, Carbohydrate Homeostasis