HNF1B Kit ELISA

N° du produit ABIN5655090

Détail du produit HNF1B Kit ELISA

Antigène: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Beta (HNF1b). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Beta (HNF1b) and analogues was observed.

Sensibilité: 0.047 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta (HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta (HNF1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HNF1B (HNF1 Homeobox B (HNF1B))

Autre désignation: Hepatocyte Nuclear Factor 1 Beta (HNF1B Produits)

Synonymes: FJHN Kit ELISA, HNF-1B Kit ELISA, HNF1beta Kit ELISA, HNF2 Kit ELISA, HPC11 Kit ELISA, LF-B3 Kit ELISA, LFB3 Kit ELISA, MODY5 Kit ELISA, TCF-2 Kit ELISA, TCF2 Kit ELISA, VHNF1 Kit ELISA, HNF-1b Kit ELISA, HNF1B Kit ELISA, AI385728 Kit ELISA, AI987804 Kit ELISA, HNF-1Beta Kit ELISA, Hnf1beta Kit ELISA, Tcf-2 Kit ELISA, Tcf2 Kit ELISA, vHNF1 Kit ELISA, vHNF-1 Kit ELISA, chunp6877 Kit ELISA, hnf1b Kit ELISA, mst Kit ELISA, tcf2 Kit ELISA, vhnf1 Kit ELISA, lfb3 Kit ELISA, hnf-1beta Kit ELISA, hnf1-beta Kit ELISA, hnf1beta Kit ELISA, HNF1g Kit ELISA, wu:fc23f06 Kit ELISA, HNF1 homeobox B Kit ELISA, HNF1 homeobox Ba Kit ELISA, HNF1 homeobox B S homeolog Kit ELISA, HNF1 homeobox B L homeolog Kit ELISA, HNF1 homeobox Bb Kit ELISA, HNF1B Kit ELISA, Hnf1b Kit ELISA, hnf1ba Kit ELISA, hnf1b.S Kit ELISA, hnf1b.L Kit ELISA, hnf1bb Kit ELISA

Sujet:

Gene Name: Hepatocyte Nuclear Factor 1 Beta

Gene Aliases: FJHN, TCF2, HNF1beta, HNF2, LFB3, LF-B3, MODY5, VHNF1, Transcription Factor 2,Hepatic, Variant Hepatic Nuclear Factor, Homeoprotein LFB3

ID gène: 6928

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation