HNF1B Kit CLIA

N° du produit ABIN5655091

Détail du produit HNF1B Kit CLIA

Antigène: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivité: Rat

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Beta (HNF1b). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Beta (HNF1b) and analogues was observed.

Sensibilité: 6.36 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 1 Beta (HNF1b) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HNF1B (HNF1 Homeobox B (HNF1B))

Autre désignation: Hepatocyte Nuclear Factor 1 Beta (HNF1B Produits)

Synonymes: FJHN Kit CLIA, HNF-1B Kit CLIA, HNF1beta Kit CLIA, HNF2 Kit CLIA, HPC11 Kit CLIA, LF-B3 Kit CLIA, LFB3 Kit CLIA, MODY5 Kit CLIA, TCF-2 Kit CLIA, TCF2 Kit CLIA, VHNF1 Kit CLIA, HNF-1b Kit CLIA, HNF1B Kit CLIA, AI385728 Kit CLIA, AI987804 Kit CLIA, HNF-1Beta Kit CLIA, Hnf1beta Kit CLIA, Tcf-2 Kit CLIA, Tcf2 Kit CLIA, vHNF1 Kit CLIA, vHNF-1 Kit CLIA, chunp6877 Kit CLIA, hnf1b Kit CLIA, mst Kit CLIA, tcf2 Kit CLIA, vhnf1 Kit CLIA, lfb3 Kit CLIA, hnf-1beta Kit CLIA, hnf1-beta Kit CLIA, hnf1beta Kit CLIA, HNF1g Kit CLIA, wu:fc23f06 Kit CLIA, HNF1 homeobox B Kit CLIA, HNF1 homeobox Ba Kit CLIA, HNF1 homeobox B S homeolog Kit CLIA, HNF1 homeobox B L homeolog Kit CLIA, HNF1 homeobox Bb Kit CLIA, HNF1B Kit CLIA, Hnf1b Kit CLIA, hnf1ba Kit CLIA, hnf1b.S Kit CLIA, hnf1b.L Kit CLIA, hnf1bb Kit CLIA

Sujet:

Gene Name: Hepatocyte Nuclear Factor 1 Beta

Gene Aliases: FJHN, TCF2, HNF1beta, HNF2, LFB3, LF-B3, MODY5, VHNF1, Transcription Factor 2,Hepatic, Variant Hepatic Nuclear Factor, Homeoprotein LFB3

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation