HMGA1 Kit ELISA

N° du produit ABIN5655127

Détail du produit HMGA1 Kit ELISA

Antigène: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of High Mobility Group AT Hook Protein 1 (HMGA1). No significant cross-reactivity or interference between High Mobility Group AT Hook Protein 1 (HMGA1) and analogues was observed.

Sensibilité: 0.124 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group AT Hook Protein 1 (HMGA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group AT Hook Protein 1 (HMGA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group AT Hook Protein 1 (HMGA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group AT Hook Protein 1 (HMGA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group AT Hook Protein 1 (HMGA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group AT Hook Protein 1 (HMGA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Autre désignation: High Mobility Group AT Hook Protein 1 (HMGA1 Produits)

Synonymes: HMG-R Kit ELISA, HMGA1A Kit ELISA, HMGIY Kit ELISA, Hmgi Kit ELISA, Hmgiy Kit ELISA, hmga1 Kit ELISA, hmgiy Kit ELISA, wu:fb12b06 Kit ELISA, wu:fb18f11 Kit ELISA, wu:fc19f01 Kit ELISA, wu:fi38b05 Kit ELISA, zgc:85677 Kit ELISA, AL023995 Kit ELISA, Hmga1a Kit ELISA, Hmga1b Kit ELISA, Hmgy Kit ELISA, HMG-I(Y) Kit ELISA, HMG-Y Kit ELISA, HMGA1 Kit ELISA, zgc:153948 Kit ELISA, high mobility group AT-hook 1 Kit ELISA, high mobility group protein HMG-I/HMG-Y Kit ELISA, high mobility group AT-hook 1a Kit ELISA, high mobility group AT-hook 1b Kit ELISA, HMGA1 Kit ELISA, Hmga1 Kit ELISA, TSC_c24220 Kit ELISA, hmga1a Kit ELISA, LOC705105 Kit ELISA, hmga1b Kit ELISA

Sujet:

Gene Name: High Mobility Group AT Hook Protein 1

Gene Aliases: HMG-R, HMGIY, High mobility group protein A1, High mobility group protein R, High mobility group protein HMG-I/HMG-Y

ID gène: 3159

UniProt: P17096

Pathways: Nuclear Hormone Receptor Binding, Autophagy