Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

UBE2K Kit ELISA

UBE2K Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5655204
  • Antigène Voir toutes UBE2K Kits ELISA
    UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Huntingtin Interacting Protein 2 (HIP2). No significant cross-reactivity or interference between Huntingtin Interacting Protein 2 (HIP2) and analogues was observed.
    Sensibilité
    0.057 ng/mL
    Featured
    Discover our best selling UBE2K Kit ELISA
    Top Product
    Discover our top product UBE2K Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Huntingtin Interacting Protein 2 (HIP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Huntingtin Interacting Protein 2 (HIP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Huntingtin Interacting Protein 2 (HIP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Huntingtin Interacting Protein 2 (HIP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Huntingtin Interacting Protein 2 (HIP2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Huntingtin Interacting Protein 2 (HIP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes UBE2K Kits ELISA
    UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))
    Autre désignation
    Huntingtin Interacting Protein 2 (UBE2K Produits)
    Synonymes
    E2-25K Kit ELISA, HIP2 Kit ELISA, HYPG Kit ELISA, LIG Kit ELISA, UBC1 Kit ELISA, E2-25k Kit ELISA, Hip2 Kit ELISA, Hypg Kit ELISA, Lig Kit ELISA, e2-25k Kit ELISA, hip2 Kit ELISA, hypg Kit ELISA, lig Kit ELISA, AW492011 Kit ELISA, D5Ertd601e Kit ELISA, ube2k Kit ELISA, zgc:103472 Kit ELISA, zgc:110791 Kit ELISA, ubiquitin conjugating enzyme E2 K Kit ELISA, ubiquitin-conjugating enzyme E2K Kit ELISA, ubiquitin conjugating enzyme E2 K S homeolog Kit ELISA, ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) Kit ELISA, ubiquitin-conjugating enzyme E2Ka (UBC1 homolog, yeast) Kit ELISA, UBE2K Kit ELISA, Ube2k Kit ELISA, ube2k.S Kit ELISA, ube2k Kit ELISA, ube2kb Kit ELISA, ube2ka Kit ELISA
    Sujet

    Gene Name: Huntingtin Interacting Protein 2

    Gene Aliases: HYPG, LIG, UBE2K, Ubiquitin-Conjugating Enzyme E2K, Ubiquitin carrier protein, Ubiquitin-protein ligase

    ID gène
    3093
    UniProt
    P61086
Support technique