IL-33 Kit ELISA

N° du produit ABIN5655959

Détail du produit IL-33 Kit ELISA

Antigène: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.8 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Interleukin 33 (IL33). No significant cross-reactivity or interference between Interleukin 33 (IL33) and analogues was observed.

Sensibilité: 3.0 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 33 (IL33). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 33 (IL33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 33 (IL33), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 33 (IL33) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 33 (IL33) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 33 (IL33) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IL-33 (IL33) (Interleukin 33 (IL33))

Autre désignation: Interleukin 33 (IL33 Produits)

Synonymes: IL33 Kit ELISA, C9orf26 Kit ELISA, DVS27 Kit ELISA, IL1F11 Kit ELISA, NF-HEV Kit ELISA, NFEHEV Kit ELISA, RP11-575C20.2 Kit ELISA, 9230117N10Rik Kit ELISA, Il-33 Kit ELISA, Il1f11 Kit ELISA, RGD1311155 Kit ELISA, interleukin 33 Kit ELISA, IL33 Kit ELISA, Il33 Kit ELISA

Sujet:

Gene Name: Interleukin 33

Gene Aliases: DV27, C9ORF26, IL1F11, NFHEV, Interleukin-1 Family, Member 11, Nuclear factor from high endothelial venules

ID gène: 361749

UniProt: Q66H70

Pathways: Production of Molecular Mediator of Immune Response