KPNB1 Kit ELISA

N° du produit ABIN5656173

Détail du produit KPNB1 Kit ELISA

Antigène: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Karyopherin Beta (KPNb1). No significant cross-reactivity or interference between Karyopherin Beta (KPNb1) and analogues was observed.

Sensibilité: 0.28 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Beta (KPNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Karyopherin Beta (KPNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Beta (KPNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Beta (KPNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Karyopherin Beta (KPNb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Karyopherin Beta (KPNb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Autre désignation: Karyopherin Beta (KPNB1 Produits)

Synonymes: Afu1g15720 Kit ELISA, AO090005001133 Kit ELISA, imb1 Kit ELISA, impnb Kit ELISA, ipo1 Kit ELISA, ipob Kit ELISA, ntf97 Kit ELISA, IMB1 Kit ELISA, IPO1 Kit ELISA, IPOB Kit ELISA, Impnb Kit ELISA, NTF97 Kit ELISA, AA409963 Kit ELISA, importin beta-1 subunit Kit ELISA, Importin beta-1 subunit Kit ELISA, karyopherin (importin) beta 1 L homeolog Kit ELISA, importin beta 1 Kit ELISA, karyopherin subunit beta 1 Kit ELISA, karyopherin (importin) beta 1 Kit ELISA, AFUA_1G15720 Kit ELISA, AOR_1_1974174 Kit ELISA, Bm1_57540 Kit ELISA, PMAA_065830 Kit ELISA, AFLA_083750 Kit ELISA, TSTA_085870 Kit ELISA, BDBG_07509 Kit ELISA, MCYG_00039 Kit ELISA, kpnb1.L Kit ELISA, LOC100533387 Kit ELISA, KPNB1 Kit ELISA, Kpnb1 Kit ELISA

Sujet:

Gene Name: Karyopherin Beta

Gene Aliases: IMB1, IPOB, Impnb, Impnb, IPO1, NTF97, PTAC97, Importin subunit beta-1, Importin-90, Nuclear factor p97, Pore targeting complex 97 kDa subunit

ID gène: 3837

UniProt: Q14974

Pathways: Protein targeting to Nucleus