KRT20 Kit ELISA

N° du produit ABIN5656193

Détail du produit KRT20 Kit ELISA

Antigène: KRT20 (Keratin 20 (KRT20))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Keratin 20 (KRT20). No significant cross-reactivity or interference between Keratin 20 (KRT20) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 20 (KRT20). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 20 (KRT20). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 20 (KRT20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 20 (KRT20) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 20 (KRT20) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 20 (KRT20) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KRT20 (Keratin 20 (KRT20))

Autre désignation: Keratin 20 (KRT20 Produits)

Synonymes: KRT20 Kit ELISA, CD20 Kit ELISA, CK-20 Kit ELISA, CK20 Kit ELISA, K20 Kit ELISA, KRT21 Kit ELISA, Krt21 Kit ELISA, 9030623C06Rik Kit ELISA, keratin 20 Kit ELISA, KRT20 Kit ELISA, Krt20 Kit ELISA

Sujet:

Gene Name: Keratin 20

Gene Aliases: CK20, Cytokeratin 20, Protein IT, Keratin, type I cytoskeletal 20

ID gène: 54474

UniProt: P35900