KRT6A Kit ELISA

N° du produit ABIN5656198

Détail du produit KRT6A Kit ELISA

Antigène: KRT6A (Keratin 6A (KRT6A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Keratin 6A (KRT6A). No significant cross-reactivity or interference between Keratin 6A (KRT6A) and analogues was observed.

Sensibilité: 6.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 6A (KRT6A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 6A (KRT6A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 6A (KRT6A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 6A (KRT6A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KRT6A (Keratin 6A (KRT6A))

Autre désignation: Keratin 6A (KRT6A Produits)

Synonymes: CK6A Kit ELISA, CK6C Kit ELISA, CK6D Kit ELISA, K6A Kit ELISA, K6C Kit ELISA, K6D Kit ELISA, KRT6C Kit ELISA, KRT6D Kit ELISA, 2310016L08Rik Kit ELISA, Krt2-6a Kit ELISA, Krt2-6c Kit ELISA, MK6a Kit ELISA, mK6[a] Kit ELISA, CK-6A Kit ELISA, Krt6a Kit ELISA, KRT6A Kit ELISA, keratin 6A Kit ELISA, similar to keratin complex 2, basic, gene 6a Kit ELISA, keratin, type II cytoskeletal 6A Kit ELISA, KRT6A Kit ELISA, Krt6a Kit ELISA, LOC683313 Kit ELISA, LOC101112304 Kit ELISA

Sujet:

Gene Name: Keratin 6A

Gene Aliases: K6A, KRT6C, KRT6D, CK6C, K6C, CK6D, K6D, Keratin 6C, Keratin 6D, Keratin, type II cytoskeletal 6A, Type-II keratin Kb6

ID gène: 3853

UniProt: P02538