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KIF2A Kit ELISA

KIF2A Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
N° du produit ABIN5656222
  • Antigène Voir toutes KIF2A Kits ELISA
    KIF2A (Kinesin Heavy Chain Member 2A (KIF2A))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Kinesin 2 (KNS2). No significant cross-reactivity or interference between Kinesin 2 (KNS2) and analogues was observed.
    Sensibilité
    0.106 ng/mL
    Top Product
    Discover our top product KIF2A Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin 2 (KNS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin 2 (KNS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin 2 (KNS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin 2 (KNS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kinesin 2 (KNS2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kinesin 2 (KNS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes KIF2A Kits ELISA
    KIF2A (Kinesin Heavy Chain Member 2A (KIF2A))
    Autre désignation
    Kinesin 2 (KIF2A Produits)
    Synonymes
    HK2 Kit ELISA, KIF2 Kit ELISA, C530030B14Rik Kit ELISA, Kif2 Kit ELISA, Kns2 Kit ELISA, M-kinesin Kit ELISA, kif2-a Kit ELISA, fj55b02 Kit ELISA, wu:fj55b02 Kit ELISA, kinesin family member 2A Kit ELISA, kinesin heavy chain member 2A L homeolog Kit ELISA, kinesin heavy chain member 2A Kit ELISA, zgc:103670 Kit ELISA, Kinesin-2 Kit ELISA, KIF2A Kit ELISA, Kif2a Kit ELISA, kif2a.L Kit ELISA, zgc:103670 Kit ELISA, GL50803_17333 Kit ELISA, GL50803_16456 Kit ELISA
    Sujet

    Gene Name: Kinesin 2

    Gene Aliases: KLC, KLC1, KNS2A, hKLC1S, hKLC1N, hKLC1P, hKLC1G, hKLC1R, hKLC1J, hKLC1B, Kinesin Light Chain 1

    ID gène
    3831
    UniProt
    Q07866
    Pathways
    Dynamique des Microtubules, Ribonucleoprotein Complex Subunit Organization
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