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Laminin alpha 5 Kit ELISA

LAMA5 Reactivité: Souris Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5656315
  • Antigène Voir toutes Laminin alpha 5 (LAMA5) Kits ELISA
    Laminin alpha 5 (LAMA5) (Laminin, alpha 5 (LAMA5))
    Reactivité
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78.12 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78.12 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Laminin Alpha 5 (LAMa5). No significant cross-reactivity or interference between Laminin Alpha 5 (LAMa5) and analogues was observed.
    Sensibilité
    33 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 5 (LAMa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 5 (LAMa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 5 (LAMa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Alpha 5 (LAMa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Alpha 5 (LAMa5) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Alpha 5 (LAMa5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Laminin alpha 5 (LAMA5) Kits ELISA
    Laminin alpha 5 (LAMA5) (Laminin, alpha 5 (LAMA5))
    Autre désignation
    Laminin Alpha 5 (LAMA5 Produits)
    Synonymes
    cb402 Kit ELISA, sb:cb402 Kit ELISA, im:7138247 Kit ELISA, wu:fb81a10 Kit ELISA, AA408760 Kit ELISA, AA408762 Kit ELISA, AI853660 Kit ELISA, [a]5 Kit ELISA, laminin-511 Kit ELISA, mKIAA0533 Kit ELISA, laminin subunit alpha 5 Kit ELISA, laminin, alpha 5 Kit ELISA, LAMA5 Kit ELISA, lama5 Kit ELISA, Lama5 Kit ELISA
    Sujet

    Gene Name: Laminin Alpha 5

    Gene Aliases: LAM-A5

    ID gène
    16776
    UniProt
    Q61001
    Pathways
    Cell-Cell Junction Organization
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