LAP Kit ELISA

N° du produit ABIN5656379

Détail du produit LAP Kit ELISA

Antigène: LAP (Leucine Aminopeptidase (LAP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Leucine Aminopeptidase (LAP). No significant cross-reactivity or interference between Leucine Aminopeptidase (LAP) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Aminopeptidase (LAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Aminopeptidase (LAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Aminopeptidase (LAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucine Aminopeptidase (LAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Aminopeptidase (LAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Aminopeptidase (LAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LAP (Leucine Aminopeptidase (LAP))

Autre désignation: Leucine Aminopeptidase (LAP Produits)

Synonymes: DDBDRAFT_0218188 Kit ELISA, DDBDRAFT_0231024 Kit ELISA, DDB_0218188 Kit ELISA, DDB_0231024 Kit ELISA, 18.m06372 Kit ELISA, CALM Kit ELISA, CLTH Kit ELISA, LAP Kit ELISA, Calm Kit ELISA, leucine aminopeptidase Kit ELISA, leucine aminopeptidase, putative Kit ELISA, phosphatidylinositol binding clathrin assembly protein Kit ELISA, PVX_118180 Kit ELISA, CpipJ_CPIJ002841 Kit ELISA, lap Kit ELISA, pepA Kit ELISA, NAEGRDRAFT_78195 Kit ELISA, TP04_0561 Kit ELISA, BBOV_II004450 Kit ELISA, PKH_126020 Kit ELISA, NAEGRDRAFT_82309 Kit ELISA, PICALM Kit ELISA, Picalm Kit ELISA

Sujet:

Gene Name: Leucine Aminopeptidase

Gene Aliases: LAPEP, LAP3, PEPS, Peptidase S, Leucyl Aminopeptidase, Cytosol aminopeptidase, Proline aminopeptidase, Prolyl aminopeptidase

ID gène: 51056

UniProt: P28838