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LRRC3C Kit CLIA

Ce kit ELISA Chemiluminescent est conçu pour la mesure quantitative de Humain LRRC3C.
N° du produit ABIN5656386

Aperçu rapide pour LRRC3C Kit CLIA (ABIN5656386)

Antigène

Tous les produits LRRC3C
LRRC3C (Leucine Rich Repeat Containing 3C (LRRC3C))

Reactivité

  • 1
  • 1
Humain

Méthode de détection

Chemiluminescent

Type de méthode

Sandwich ELISA

Gamme de detection

312.5 pg/mL - 20000 pg/mL

Application

ELISA

Type d'échantillon

Tissue Homogenate
  • Seuil minimal de détection

    312.5 pg/mL

    Analytical Method

    Quantitative

    Specificité

    This assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat Containing Protein 3C (LRRC3C). No significant cross-reactivity or interference between Leucine Rich Repeat Containing Protein 3C (LRRC3C) and analogues was observed.

    Sensibilité

    13 pg/mL
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test

    2 - 3 h

    Plaque

    Pre-coated

    Protocole

    The microplate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Leucine Rich Repeat Containing Protein 3C (LRRC3C) level in the sample or standard.,

    Précision du teste

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Conseil sur la manipulation

    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

    Date de péremption

    4-8 months
  • Antigène Tous les produits LRRC3C

    LRRC3C (Leucine Rich Repeat Containing 3C (LRRC3C))

    Autre désignation

    Leucine Rich Repeat Containing Protein 3C

    Sujet

    Gene Name: Leucine Rich Repeat Containing Protein 3C

    ID gène

    100505591

    UniProt

    A6NJW4
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